Protein Info for PP_4820 in Pseudomonas putida KT2440

Annotation: tRNA-dihydrouridine synthase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 337 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 6 to 321 (316 residues), 426.9 bits, see alignment E=2.5e-132 PF01207: Dus" amino acids 16 to 322 (307 residues), 363.5 bits, see alignment E=4.4e-113

Best Hits

Swiss-Prot: 100% identical to DUSB_PSEPK: tRNA-dihydrouridine synthase B (dusB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K05540, tRNA-dihydrouridine synthase B [EC: 1.-.-.-] (inferred from 100% identity to ppf:Pput_4695)

MetaCyc: 63% identical to tRNA-dihydrouridine synthase B (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DK5 at UniProt or InterPro

Protein Sequence (337 amino acids)

>PP_4820 tRNA-dihydrouridine synthase B (Pseudomonas putida KT2440)
MSAVRIGQYTLRNNLILAPMAGVTDQPFRTLCQRLGAGMVVSEMVSSDMSLWNSRKSSLR
RIHEGDPEPRSVQIAGGDAQMMAAAAKANVEAGAQIIDINMGCPAKKVCNKAAGSALLRD
EALVSEILHAVVSAVDVPVTLKIRTGWDRANKNGLNVAKIAEQAGIQALAVHGRTRADLY
TGEAEYDTIGAIKQAVSIPVFANGDITSPEKARAVLETTGVDGLLIGRAAQGRPWIFREI
EHYLRTGEHLPAPQLDEVERILLEHLAALHAFYGDVMGVRIARKHVGWYLATRPGGKEFR
ARFNALEDTQAQCANVRAFFSERRQSLETEDGQGVAA