Protein Info for PP_4811 in Pseudomonas putida KT2440

Annotation: Gamma-glutamyl phosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PROA_PSEPK: Gamma-glutamyl phosphate reductase (proA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 100% identity to ppf:Pput_4686)

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

L-citrulline biosynthesis (7/8 steps found)
L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
L-ornithine biosynthesis II (3/3 steps found)
superpathway of L-citrulline metabolism (9/12 steps found)
L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DL4 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PP_4811 Gamma-glutamyl phosphate reductase (Pseudomonas putida KT2440)
MTESVLDYMTRLGRAAREASRVIGRASTAQKNRALQAAADALDAARAELTAANELDLAAG
RASGLEPALLDRLALTPARIDGMITGLRQVASLPDPVGAIRDMSYRPSGIQVGKMRTPLG
VIGIIYESRPNVTIDAASLCLKSGNATILRGGSEAIHSNRAIATCIQRGLAEAGLPAAVV
QVVETTDREAVGALISMPEFVDVIVPRGGRGLIERISRDARVPVIKHLDGICHIYVSQHA
DLDKAWNVAFNAKTYRYGICGAMETLLVDQQVAERFLPEMARRFVEKGVELRGCERTQAI
ISAKPATEADWHTEYLDAILSIRVVDGLNQAIEHINHYGSHHTDSIISEHQGEARQFMAE
VDSASVMLNTPTCFADGFEYGLGAEIGISTDKLHARGPVGLEGLTCEKYVVIGDGQLRGQ
GSC