Protein Info for PP_4808 in Pseudomonas putida KT2440

Annotation: 23S rRNA m3psi1915 methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 172 TIGR00246: rRNA large subunit m3Psi methyltransferase RlmH" amino acids 18 to 172 (155 residues), 207.8 bits, see alignment E=4.1e-66 PF02590: SPOUT_MTase" amino acids 19 to 171 (153 residues), 159.8 bits, see alignment E=2.7e-51

Best Hits

Swiss-Prot: 99% identical to RLMH_PSEPK: Ribosomal RNA large subunit methyltransferase H (rlmH) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00783, hypothetical protein (inferred from 91% identity to pfo:Pfl01_4971)

MetaCyc: 56% identical to 23S rRNA m3psi1915 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11592 [EC: 2.1.1.177]

Predicted SEED Role

"LSU m3Psi1915 methyltransferase RlmH" in subsystem Ribosome biogenesis bacterial

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.177

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DL7 at UniProt or InterPro

Protein Sequence (172 amino acids)

>PP_4808 23S rRNA m3psi1915 methyltransferase (Pseudomonas putida KT2440)
MPTSTPTSSKTANKESAVRLRLIAVGSRMPKWVEEGWHEYAKRLPAELSLELVEIPLNTR
GKNADVARLIRQEGEAMLSKVQPGERIVTLEVHGKPWSTEQLATELDRWRLDARTVNLMV
GGPEGLAPEVCARAEQRWSLSPLTLPHPLVRILIGEQIYRAWTVLSGHPYHK