Protein Info for PP_4804 in Pseudomonas putida KT2440

Annotation: RlpA-like lipoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF03330: DPBB_1" amino acids 100 to 189 (90 residues), 89.2 bits, see alignment E=1.7e-29 TIGR00413: rare lipoprotein A" amino acids 102 to 195 (94 residues), 142.4 bits, see alignment E=9.6e-46 PF05036: SPOR" amino acids 256 to 323 (68 residues), 49.7 bits, see alignment E=3.7e-17

Best Hits

Swiss-Prot: 70% identical to RLPA_PSEAE: Endolytic peptidoglycan transglycosylase RlpA (rlpA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03642, rare lipoprotein A (inferred from 100% identity to ppu:PP_4804)

Predicted SEED Role

"Rare lipoprotein A precursor" in subsystem Peptidoglycan Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DM1 at UniProt or InterPro

Protein Sequence (333 amino acids)

>PP_4804 RlpA-like lipoprotein (Pseudomonas putida KT2440)
MRAIISGNAFKLLTCLAVGVALASCSSNRPAPKSSGNVVRSQPGLDINRAHKDGAPWWDV
DVNKIPDATPTVHTGNYKANPYTVLGKTYYPMQDSRNYRAEGTASWYGTKFHGQNTANGE
LYDLYGMSAAHKTLPLPAYVRVTNLANGRSVILRVNDRGPFYSDRIIDLSYAAAKKLGYA
EIGTAHVRVEGIDPQQWWAQRGQTPPMVLKEPQVAQTQAIPASTGRVEQWTPPPQQHAAP
VVPVQVGGNNVPGGNGGSFLQVGAFANPDAAELLRAKLSTMVNAPVFISSIVRNQQTLHR
VRLGPIGSQGEIQQAQDSIRLANLGQAKLVTAD