Protein Info for PP_4794 in Pseudomonas putida KT2440

Annotation: Leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR00396: leucine--tRNA ligase" amino acids 6 to 867 (862 residues), 1146.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 225 (211 residues), 98.9 bits, see alignment E=5.9e-32 amino acids 422 to 580 (159 residues), 33.6 bits, see alignment E=3.8e-12 amino acids 626 to 660 (35 residues), 28 bits, see alignment (E = 2e-10) PF09334: tRNA-synt_1g" amino acids 39 to 171 (133 residues), 71.5 bits, see alignment E=1.2e-23 PF13603: tRNA-synt_1_2" amino acids 221 to 407 (187 residues), 241 bits, see alignment E=1.4e-75 PF08264: Anticodon_1" amino acids 707 to 831 (125 residues), 68.6 bits, see alignment E=1.1e-22

Best Hits

Swiss-Prot: 81% identical to SYL_AZOVD: Leucine--tRNA ligase (leuS) from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 81% identity to avn:Avin_09000)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DN1 at UniProt or InterPro

Protein Sequence (868 amino acids)

>PP_4794 Leucine--tRNA ligase (Pseudomonas putida KT2440)
MHEQYTPRDIEAAAQKFWDEQQSFAVTEQPGKDTYYCLSMFPYPSGKLHMGHVRNYTIGD
VIARYQRMLGKNVLQPMGWDAFGMPAENAAMKNNVAPAKWTYENIDYMKTQLKSLGLAID
WAREVTTCKPDYYRWEQWLFTRLFEKGIIYRKNGTVNWDPADQTVLANEQVIDGRGWRSG
ALIEKREIPMYYFRITDYADELLESLDELPGWPEQVKTMQRNWIGKSRGMEVQFPYDKAS
IGHEGTLKVFTTRPDTLMGATYVAVAAEHPLATQAAQGNPALQAFIDECKSGSVAEADMA
TQEKKGMATSLLVEHPLTGEKLPVWVANYVLMHYGDGAVMAVPAHDERDFEFAHKYNLPV
KAVVRTSAGDEVGSEWQAAYGEHGQLINSAEFDGLDFAGAFDAIEAALIRKELGKSRTQF
RLRDWGISRQRYWGCPIPIIHCPSCGDVPVPEDQLPVTLPENVVPDGAGSPLARMPEFYE
CSCPKCGAAAKRETDTMDTFVESSWYFARYASPNYDKGLVDPKAANHWLPVDQYIGGIEH
AILHLLYARFFHKLMRDEGLVTSNEPFKNLLTQGMVVAETYYRVASNGGKDWFNPADVEI
ERDAKAKIIGARLKTDGLPVEIGGTEKMSKSKNNGVDPQSMIEAYGADTCRLFMMFASPP
DMSLEWSDSGVEGASRFLRRVWRLAQAHVSQGLPGKLDVAALDDAQKVIRRAIHAAIKQA
STDVGQFHKFNTAIAQVMTVMNVLEKAPQATEQDRALLQEGLEAVTLLLAPITPHISHAL
WQHLGHAGSVIDAAWPSVDEQALVQDSITLVVQVNGKLRGQVEMPAAASREEVEAAARSN
ENVLRFIDGLTIRKVIVVPGKLVNIVAN