Protein Info for PP_4737 in Pseudomonas putida KT2440

Annotation: D-lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 PF01565: FAD_binding_4" amino acids 41 to 175 (135 residues), 119.5 bits, see alignment E=2.8e-38 PF02913: FAD-oxidase_C" amino acids 267 to 513 (247 residues), 219.2 bits, see alignment E=2.3e-68 PF13183: Fer4_8" amino acids 537 to 608 (72 residues), 40.7 bits, see alignment 8.6e-14 PF12838: Fer4_7" amino acids 539 to 608 (70 residues), 35.4 bits, see alignment 3.6e-12 PF13534: Fer4_17" amino acids 539 to 609 (71 residues), 32.5 bits, see alignment 3e-11 PF02754: CCG" amino acids 705 to 794 (90 residues), 19.6 bits, see alignment E=2.4e-07 amino acids 833 to 896 (64 residues), 13.4 bits, see alignment 2.1e-05

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_4603)

Predicted SEED Role

"Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DT2 at UniProt or InterPro

Protein Sequence (936 amino acids)

>PP_4737 D-lactate dehydrogenase (Pseudomonas putida KT2440)
MSLPAAFLRDAERLIPAERRFDDPTSTLAFGTDASFYRLIPKLVVRVESEDEVVGLIKLA
QRERVPVTFRAAGTSLSGQAISDSVLIVLGDNWNGREIRRQGEQIRLQPGVIGAQANAWL
APFGRKIGPDPASINACKIGGIVANNASGMCCGTAQNTYHTLAGLRLVLADGTRLDSEDP
ASVAAFESSHAELLEALARLGRETRANTALAERIRHKYRLKNTTGLSLNALVDYDQPLDI
LQHLLVGSEGTLGFISAVTYDTVPDHPHKASALLVFPSVESCCRAVPVLKQQPVSAVELL
DRRSLRSVQNMPGMPLWVKGLSDNACALLIESRAASQSLLHEQLQQIMASIADFPLEQQV
DFSEDPAVYNQLWKIRKDTFPAVGAVRQTGTTVIIEDVTFPVEQLAEGVNRLIQLFDKHH
YDEAIIFGHALEGNLHFVFTQGFNSAEEVARYQAFMDDVAQLVAVEFGGSLKAEHGTGRN
MAPFVELEWGHDAYQLMWKLKRLLDPNGILNPDVVLSEDPDIHLKNLKPLPAADKIVDKC
IECGFCEPVCPSKGLTLSPRQRIVMWRDIQAKQRAGIDTRELMQSYQYQGIDTCAATGLC
AQRCPVGINTGELVKKLRSQAADHAKTADWLAEHFHTALSGARLTLTAANTARKLLGAPR
LGRLSASLSKASKGRLPQWTPAMPQPLRPLAFGATSNDARPRVVYLAACVSRVMGPAYAD
REQSSLLDKTRALLEKAGYQVVFPDNADSLCCGQPFASKGYPEQAEHKRQELITALLHAS
RGGLDPIYCDTSPCTLRLVQDLSDTRLDLYDPVRFIRTHLLDRLEFTPQDEPVAVHVTCS
TQHLGESQALIDLARRCSKQVVIPEGIHCCGFAGDKGFTTPELNAHSLRSLKDAVQYCSE
GISTSRTCEIGLSSHSGIDYHGLVYLVDRVTRPRSL