Protein Info for PP_4728 in Pseudomonas putida KT2440

Annotation: Protein GrpE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 PF01025: GrpE" amino acids 13 to 172 (160 residues), 181.3 bits, see alignment E=6.3e-58

Best Hits

Swiss-Prot: 100% identical to GRPE_PSEPK: Protein GrpE (grpE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03687, molecular chaperone GrpE (inferred from 100% identity to ppf:Pput_4594)

Predicted SEED Role

"Heat shock protein GrpE" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DU1 at UniProt or InterPro

Protein Sequence (185 amino acids)

>PP_4728 Protein GrpE (Pseudomonas putida KT2440)
MADEQLNEKDLNVEETGAGNAADTRVLELEEQLAAAKDQALRAVADLQNVRRRAEQDVEK
AHKFALEKFSSDLLPVIDSLELALAHSSADDEHVKQIREGVELTLKMFQDTLKRYNLEAV
DPHGQPFNPEHHQAMAMQENAEVEPNSVLNVFQKGYLLNGRLLRPAMVVVSKAPSAAQPS
INEKA