Protein Info for PP_4727 in Pseudomonas putida KT2440

Annotation: Chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 956.7 bits, see alignment E=2.1e-292 PF00012: HSP70" amino acids 4 to 603 (600 residues), 884.3 bits, see alignment E=5.4e-270 PF06723: MreB_Mbl" amino acids 116 to 377 (262 residues), 43.5 bits, see alignment E=2.9e-15

Best Hits

Swiss-Prot: 100% identical to DNAK_PSEPK: Chaperone protein DnaK (dnaK) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to ppu:PP_4727)

MetaCyc: 76% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DU2 at UniProt or InterPro

Protein Sequence (641 amino acids)

>PP_4727 Chaperone protein DnaK (Pseudomonas putida KT2440)
MGKIIGIDLGTTNSCVSILENGNVKVIENAEGARTTPSIVAYANDGEILVGQSAKRQAVT
NPHNTLFAVKRLIGRRFEEDVVQKDIKLVPYKIVKANNGDAWVEAAGKEMAPPQISAEVL
KKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAYG
MDKAKGDHTVIVYDLGGGTFDVSVIEIAEVDGEHQFEVLATNGDTFLGGEDFDMRLIDYL
VDEFKKESGMDLKNDPLALQRLKEAAEKAKIELSSAQSTDVNLPYITADATGPKHLNVKI
SRAKLESLVEDLVKRTIEPCRIALKDAGIDASKIDDVILVGGQTRMPLVQKEVADFFGKE
ARKDVNPDEAVAMGAAIQGAVLAGDVKDVLLLDVSPLTLGIETMGGVMTALIEKNTTIPT
KKSQVFSTADDNQSAVTIHVLQGERKQAAQNKSLGKFDLADIPPAPRGVPQIEVTFDIDA
NGILHVGAKDKATGKTQSIVIKANSGLSDEEIERMVRDAEANAEEDRKFEELAAARNQGD
ALVHSTRKMVADAGDKVTAEEKTAIEAAVVALEAAVKGDDKAAIDAKVEELSKVSAPVAQ
KMYAEQSAEQPQGGAQQAEPEAKHDDVVDAEFEEVKDNNKQ