Protein Info for PP_4723 in Pseudomonas putida KT2440

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 5 to 1056 (1052 residues), 1566.7 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 131 to 337 (207 residues), 244.1 bits, E=5.2e-76
  • amino acids 676 to 878 (203 residues), 113.3 bits, E=5.6e-36
• PF02787: CPSase_L_D3
  • amino acids 430 to 508 (79 residues), 90.7 bits, 2.2e-29
• PF02222: ATP-grasp
  • amino acids 687 to 848 (162 residues), 36.3 bits, E=1.9e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 698 to 846 (149 residues), 24.8 bits, E=6.5e-09
• PF02655: ATP-grasp_3
  • amino acids 706 to 848 (143 residues), 30.8 bits, E=1.4e-10
• PF02142: MGS
  • amino acids 961 to 1044 (84 residues), 79.4 bits, 8.4e-26

Best Hits

Swiss-Prot: 67% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 77% identity to abo:ABO_0318)

MetaCyc: 74% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q88DU6 at UniProt or InterPro

Protein Sequence (1076 amino acids)

>PP_4723 Carbamoyl-phosphate synthase large chain (Pseudomonas putida KT2440)
MTTMPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGFRVILVNSNPATIMTD
PAMADATYIEPIKWQSVAKIIEKERPDAVLPTMGGQTALNCALDLERHGVLEKFGVEMIG
ANADTIDKAEDRSRFDKAMKDIGLECPRSGIAHSMEEANAVLEKLGFPCIIRPSFTMGGT
GGGIAYNREEFEEICTRGLDLSPTKELLIDESLIGWKEYEMEVVRDKKDNCIIVCSIENF
DPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIGVETGGSNVQFGICPNTGRMVV
IEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDELQNDITGGRTPASFEPSIDYVVT
KLPRFAFEKFPKADARLTTQMKSVGEVMAIGRTFQESLQKALRGLEVGACGLDPKVDLAS
PEAASILKRELTVPGAERIWYVADAMRSGMTCEEIFNLTGIDMWFLVQMEDLIKEEEKVK
TLALSAIDKDYMLRLKRKGFSDQRLAVLLGITDKNLRRHRHKLEVFPVYKRVDTCAAEFA
TDTAYLYSTYEEECEANPSTRDKIMILGGGPNRIGQGIEFDYCCVHAALALREDGYETIM
VNCNPETVSTDYDTSDRLYFEPLTLEDVLEVCRVEKPKGVIVHYGGQTPLKLARALEEAG
VPIIGTSPDAIDRAEDRERFQQMVQRLSLLQPPNATVRSEEEAIRAAGSIGYPLVVRPSY
VLGGRAMEIVYELDELKRYLREAVQVSNDSPVLLDHFLNCAIEMDVDAVCDGTDVVIGAI
MQHIEQAGVHSGDSACSLPPYSLSKEVQDEVRVQVKKMALELGVVGLMNVQLALQGDKIY
VIEVNPRASRTVPFVSKCIGTSLAMIAARVMAGKTLKELGFTQEIIPNFYSVKEAVFPFA
KFPGVDPILGPEMKSTGEVMGVGDSFGEAFAKAQMGASEVLPTGGTAFISVRDDDKPQVA
GVARDLIALGFEVVATAGTAKVIEAAGLKVRRVNKVTEGRPHVVDMIKNDEVSLIINTTE
GRQSIADSYSIRRNALQHKIYCTTTIAAGEAICEALKFGPEKTVRRLQDLHAGLKA