Protein Info for PP_4713 in Pseudomonas putida KT2440

Annotation: transcription termination/antitermination L factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 PF08529: NusA_N" amino acids 4 to 125 (122 residues), 129.1 bits, see alignment E=2e-41 TIGR01953: transcription termination factor NusA" amino acids 5 to 344 (340 residues), 410.2 bits, see alignment E=6.1e-127 PF00575: S1" amino acids 135 to 197 (63 residues), 30.1 bits, see alignment E=1e-10 PF13184: KH_5" amino acids 232 to 299 (68 residues), 95.3 bits, see alignment E=3.9e-31 TIGR01954: transcription termination factor NusA, C-terminal duplication" amino acids 367 to 415 (49 residues), 70.3 bits, see alignment 1.1e-23 amino acids 442 to 491 (50 residues), 66 bits, see alignment 2.5e-22 PF14520: HHH_5" amino acids 434 to 488 (55 residues), 37.8 bits, see alignment 4.4e-13

Best Hits

Swiss-Prot: 64% identical to NUSA_SHIFL: Transcription termination/antitermination protein NusA (nusA) from Shigella flexneri

KEGG orthology group: K02600, N utilization substance protein A (inferred from 100% identity to ppg:PputGB1_4713)

Predicted SEED Role

"Transcription termination protein NusA" in subsystem NusA-TFII Cluster or Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DV6 at UniProt or InterPro

Protein Sequence (493 amino acids)

>PP_4713 transcription termination/antitermination L factor (Pseudomonas putida KT2440)
MSKEVLLVVESVSNEKGVPPGVIFEALEVALATATKKRFEDEVDLRVEINRHTGSYETFR
RWTVVDEADLDDPAIETWLDKIKDTHPEAKVGDVIEEKIESIEFGRIAAQTAKQVIVQKV
REAERAQVVDAYRERVGEIISGTVKKVTRDNVIVDLGNNAEALLAREDIIPRETFRVGVR
LRALLKEIRTENRGPQLILSRTAPQMLIELFRIEVPEIAEGLIEVMAASRDPGSRAKIAV
RSKDKRIDPQGACIGMRGSRVQAVSGELGGERVDIVLWDDNPAQFVINAMSPAEVAAIIV
DEDAHAMDIAVAEDNLAQAIGRGGQNVRLASQLTGWTLNVMTEKDIQAKQQAETGDILRN
FIEELEVDEELAQVLVDEGFTSLEEIAYVPLEEMLNIDGFDEDIVNELRARAKDRLLTKA
IATEEKLADAHPAEDLLSLEGMDKDLAAELAVRGVVNREDLAEQSIDDLLDIDGIDEERA
GKLIMAARAHWFE