Protein Info for PP_4712 in Pseudomonas putida KT2440

Annotation: Translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 PF04760: IF2_N" amino acids 1 to 51 (51 residues), 32.1 bits, see alignment 3.5e-11 amino acids 271 to 321 (51 residues), 58.5 bits, see alignment 2e-19 PF08364: IF2_assoc" amino acids 61 to 95 (35 residues), 41.1 bits, see alignment (E = 7.6e-14) TIGR00487: translation initiation factor IF-2" amino acids 265 to 846 (582 residues), 857.6 bits, see alignment E=5.6e-262 TIGR00231: small GTP-binding protein domain" amino acids 349 to 505 (157 residues), 107.4 bits, see alignment E=6.4e-35 PF00009: GTP_EFTU" amino acids 350 to 506 (157 residues), 130.9 bits, see alignment E=2e-41 PF01926: MMR_HSR1" amino acids 351 to 456 (106 residues), 40.2 bits, see alignment E=1.5e-13 PF22042: EF-G_D2" amino acids 523 to 601 (79 residues), 108.4 bits, see alignment E=7.4e-35 PF11987: IF-2" amino acids 622 to 736 (115 residues), 133 bits, see alignment E=2.3e-42

Best Hits

Swiss-Prot: 100% identical to IF2_PSEPK: Translation initiation factor IF-2 (infB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 100% identity to ppf:Pput_4577)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88DV7 at UniProt or InterPro

Protein Sequence (846 amino acids)

>PP_4712 Translation initiation factor IF-2 (Pseudomonas putida KT2440)
MTQVTVKELAQEVEAPVERLLQQMREAGLPHTDAGQVVTDNEKQTLLTHLKSSHKSKAEE
PRKITLQRKTTSTLRVAGSKSISVEVRKKKVFVQRSPEEIQAEQKRELDERRAAENAARD
KVEAEVRQRNEEQARRQAAGSAAAAPAPAAKPEPAPAAAPVAAPAPVVADAPASEDAAAR
AAERKKDETRRNESRTRDDDRRRGEAPRVSIKVKVKEKEKAPTPRAAPRTTDEESDGARR
GRGGKSKLKKRNQHGFQNPTGPVIRDVTIGETITVSELANQMSVKGAEVVKFMFKMGTPV
TINQVLDQETAQLIAEELGHKVTLVSDTALEDSLAESLKFEGQTESRAPVVTVMGHVDHG
KTSLLDYIRRAKVAAGEAGGITQHIGAYHVETDRGMVTFLDTPGHAAFTQMRARGAKATD
IVILVVAADDGVMPQTREAVQHAKAAGVPLVVAVNKIDKPGADLDRIRNELSVEGVTSED
WGGDTPFVKVSAKMGTGVDELLEAVLLQAEILELTATPTAPGRGVVVESRLDKGRGPVAT
ILVQDGTLRQGDMVLCGSNYGRVRAMLDENGKPVKEAGPSIPVEILGLDGTPEAGDELSV
VADEKKAREVALFRQGKYREVKLARAHAGKLENIFETMGQEEKKTLNIVLKTDVRGSLEA
LQGSLGGLGNDEVQVRVIGGGVGGITESDANLALASNAVLFGFNVRADAGARKIVEQEGL
DMRYYNVIYDIIEDVKKALTGMLGSDVRENILGVAEVRDVFRSPKFGAIAGCMVIEGTVY
RNRPIRVLRDDVVIFEGELESLRRFKDDASEVRSGMECGIGVKSYNDVKVGDKIEVFEKV
QVARTL