Protein Info for PP_4680 in Pseudomonas putida KT2440

Annotation: acetohydroxybutanoate synthase / acetolactate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 4 to 569 (566 residues), 854.5 bits, E=1.7e-261
• PF02776: TPP_enzyme_N
  • amino acids 5 to 118 (114 residues), 147.8 bits, E=1.8e-47
• PF00205: TPP_enzyme_M
  • amino acids 196 to 330 (135 residues), 159.6 bits, E=5.7e-51
• PF02775: TPP_enzyme_C
  • amino acids 396 to 545 (150 residues), 184.4 bits, E=1.8e-58

Best Hits

Swiss-Prot: 65% identical to ILVI_ECOLI: Acetolactate synthase isozyme 3 large subunit (ilvI) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 100% identity to ppf:Pput_4544)

MetaCyc: 65% identical to acetolactate synthase / acetohydroxybutanoate synthase, catalytic subunit (Escherichia coli K-12 substr. MG1655)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• pyruvate fermentation to (R)-acetoin I (2/3 steps found)
• pyruvate fermentation to (S)-acetoin (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• superpathway of (R,R)-butanediol biosynthesis (3/5 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of 2,3-butanediol biosynthesis (2/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Sequence Analysis Tools

See Q88DY8 at UniProt or InterPro

Protein Sequence (574 amino acids)

>PP_4680 acetohydroxybutanoate synthase / acetolactate synthase (Pseudomonas putida KT2440)
MELLSGAEMVVRFLRDEGVKHIYGYPGGALLHVYDALFKEPEVEHILVRHEQAATHMADG
YARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVILSGQVPSTMVGTDAFQETDMIG
ISRPIVKHSFMIKNPTEIPEVLKKAFYLAQSGRPGPVVVDIPKDMTNPAEKFEYVYPKKV
KLRSYSPAVRGHSGQIRKAAEMLLAAKRPVVYSGGGVILGGGSEALTEIAKSLNLPVTNT
LMGLGGFPGTDRQFLGMLGMHGSYTANMAMHHADVIFAVGARFDDRVVNGPAKFCPNAKI
IHIDIDPASISKMIKADVPIVGPVDSVLSEMLGILKEIGEQPDKAALDAWWKQIDEWRGN
GELFPYDKGDGNVIKPQKVIETLCEVTNGDAFVTSDVGQHQMFAAQYYRFNKPNRWINSG
GLGTMGFGFPAAMGVKLNFPDQDVACVTGEGSIQMNIQELSTCMQYGLPVKIVNLNNGVL
GMVRQWQDMAYNGRHSHSYVESLPDFIKLAEAYGHVGIRITSLKDLKPKLEEAFAMKDRL
VFIDIAVDRSEHVYPMQIKDGSMRDMWLSKTERT