Protein Info for PP_4657 in Pseudomonas putida KT2440

Annotation: zinc metalloprotease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 53 to 70 (18 residues), see Phobius details PF04228: Zn_peptidase" amino acids 23 to 311 (289 residues), 416.3 bits, see alignment E=3.9e-129

Best Hits

KEGG orthology group: K07054, (no description) (inferred from 100% identity to ppu:PP_4657)

Predicted SEED Role

"YpfJ protein, zinc metalloprotease superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88E11 at UniProt or InterPro

Protein Sequence (316 amino acids)

>PP_4657 zinc metalloprotease (Pseudomonas putida KT2440)
MPDTSRFPELIFVFLFHARRAFMEWRKGRRSDNVVDARGEGGGGGMRFGGGKGLGLGAIL
LIVGIGWLTGQDPLQILGQLTGQLEQQQTAPSGTSAKAPPANDQQAEFVASILGDTEDTW
KALFAQAGKQYRDPKLVLFSGQVNSACGFASAAVGPFYCPADQRVYLDMSFFREMETRFA
AAGDFAQAYVIAHEIGHHVQTLLGVSAKVDAARRAGQRMEGDNGLLVRQELQADCLAGVW
AYQAQKRLNWLEPGDVEEALNAANAIGDDRLQQQGRGRVVPDSFTHGTSAQRVRWFKIGF
AEGEVNRCDTFSARTL