Protein Info for PP_4655 in Pseudomonas putida KT2440

Annotation: protocatechuate 3,4-dioxygenase alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02423: protocatechuate 3,4-dioxygenase, alpha subunit
  • amino acids 9 to 201 (193 residues), 262.9 bits, E=9e-83
• PF00775: Dioxygenase_C
  • amino acids 41 to 192 (152 residues), 48 bits, E=5.2e-17

Best Hits

Swiss-Prot: 98% identical to PCXA_PSEPU: Protocatechuate 3,4-dioxygenase alpha chain (pcaG) from Pseudomonas putida

KEGG orthology group: K00448, protocatechuate 3,4-dioxygenase, alpha subunit [EC: 1.13.11.3] (inferred from 100% identity to ppu:PP_4655)

MetaCyc: 98% identical to protocatechuate 3,4-dioxygenase alpha subunit (Pseudomonas putida)
Protocatechuate 3,4-dioxygenase. [EC: 1.13.11.3]; 1.13.11.- [EC: 1.13.11.3]

Predicted SEED Role

"Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 1.13.11.3)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• gallate degradation II (4/5 steps found)
• 4-sulfocatechol degradation (2/4 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
• superpathway of aerobic toluene degradation (13/30 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.3

Use Curated BLAST to search for 1.13.11.3

Sequence Analysis Tools

See Q88E13 at UniProt or InterPro

Protein Sequence (201 amino acids)

>PP_4655 protocatechuate 3,4-dioxygenase alpha chain (Pseudomonas putida KT2440)
MPIELLPETPSQTAGPYVHIGLALEAAGNPTRDQEIWNCLAKPDAPGEHILLIGHVYDGN
GHLVRDSFLEVWQADANGEYQDAYNLENAFNSFGRTATTFDAGEWTLQTVKPGVVNNAAG
VPMAPHINISLFARGINIHLHTRLYFDDEAQANAKCPVLNLIEQPQRRETLIAKRCEVDG
KTAYRFDIRIQGEGETVFFDF