Protein Info for PP_4638 in Pseudomonas putida KT2440

Annotation: Methylenetetrahydrofolate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF02219: MTHFR" amino acids 5 to 238 (234 residues), 42.7 bits, see alignment E=4.9e-15 PF12225: DUF5981" amino acids 378 to 460 (83 residues), 69.9 bits, see alignment E=1.2e-23

Best Hits

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 100% identity to ppu:PP_4638)

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.20

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88E30 at UniProt or InterPro

Protein Sequence (495 amino acids)

>PP_4638 Methylenetetrahydrofolate reductase (Pseudomonas putida KT2440)
MSLLKTALREQNFVCVMEFVPKPSAERFAAMEAILARAHLCGWPMTVAIGDRVGSPLDMS
PLDALASFSNPVPALPHFSGKDRERHHLLAQLQRMDAAGLDQLLLLTGDRLPGHEPGQRP
VRYLESVAALQVARQACPHWLLGAALNPFKYHEEEGGAQYFKAEKKLAAGADFLTLQLGF
DAAKHQEAIHWMRRQASPKPMLACLMSLTHGRATMLDHVAGVTVTPSMRDMLEAETAQSK
AFAQARSVDRLALQIIGTKLMGYAGVHLSGVHTLEQLLALETRIEYWQTQVQSLEQWAPA
WHASWQMPGLPAVTFHPPQAGWRLDESRVDASFKEKARYHLMHGMHALLFSRRNSLSKAF
GWAVRQPLWGTRVGAHVLHTLERSVKRPLVGCDTCGRCRLEDTLYICPETCPKGLANGPC
GGTALNRCEFGDRECIHSVKYRTAKAVRQTAVLTERLIPCIEVETRHRSSWPQWFAGQAP
QRLSPQPLPRSQPES