Protein Info for PP_4575 in Pseudomonas putida KT2440

Annotation: Urea amidolyase-related protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 TIGR00724: biotin-dependent carboxylase uncharacterized domain" amino acids 6 to 297 (292 residues), 244 bits, see alignment E=9.8e-77 PF02626: CT_A_B" amino acids 26 to 286 (261 residues), 259 bits, see alignment E=2.9e-81

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4575)

Predicted SEED Role

"Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)" (EC 3.5.1.54)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88E91 at UniProt or InterPro

Protein Sequence (305 amino acids)

>PP_4575 Urea amidolyase-related protein (Pseudomonas putida KT2440)
MKQLMIEASTPLCQLQDAGRFGVRHLGVTQGGALDWVAMHWANWLLGNPLGAPVVEVALG
GFCVVAEQDCVLALAGADLDACVDDQPLAPWRSFALAKGQRLTLKQPKVGVRAYLAAPGG
FLGEDVLGSCATVVREALGGIDGQGAALAKHQALAFTGSNAPLREVPQALCPQFAQKPVL
DLVMGAQIGEFSGTSLFEAFNREWTLDSRADRMGIRLLGPQLVYQGAPMISEGIPLGAVQ
VPPDGQPIVLLNDRQTIGGYPRLGALTPLALAQLAQCMPGTVVRFRAVVQDEAWREQQLY
LGRWS