Protein Info for PP_4574 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05762: VWA_CoxE
  • amino acids 183 to 274 (92 residues), 29.1 bits, E=3.3e-11

Best Hits

KEGG orthology group: K09989, hypothetical protein (inferred from 100% identity to ppu:PP_4574)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

• thioredoxin pathway (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

See Q88E92 at UniProt or InterPro

Protein Sequence (392 amino acids)

>PP_4574 conserved protein of unknown function (Pseudomonas putida KT2440)
MLLNLFNEMRAAKVPVSVRELLDLHQALQKRVVFADMDEFYYLARAILVKDERHFDKFDR
AFAAYFKGLENLDRHIEALIPEDWLRKEFERSLTDEERAQIQSLGGLDKLIEAFKQRLEE
QKERHAGGNKWIGTGGTSPFGSGGFNPEGIRVGEAGKRQGKAVKVWDQREYKNLDDQVEL
GTRNIKLALRRLRKFAREGAAEELDIDGTIDHTARDAGLLNIQMRPERRNTVKLLLLFDI
GGSMDAHVKVCEELFSACKTEFKHLEYYYFHNCVYESVWKNNLRRTSERFSTFDLLHKYG
DDYKVVFVGDAAMAPYEITQPGGSVEHWNEEAGYVWIQRFMEKFRKVIWINPYPKQAWEY
TASTHLVRDLIEDKMYPLTLQGLEDGMRYLSK