Protein Info for PP_4565 in Pseudomonas putida KT2440

Annotation: Isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 571 PF20423: AceK_regulatory" amino acids 11 to 312 (302 residues), 341.5 bits, see alignment E=4.1e-106 PF06315: AceK_kinase" amino acids 313 to 567 (255 residues), 396 bits, see alignment E=8e-123

Best Hits

Swiss-Prot: 100% identical to ACEK_PSEP1: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 100% identity to ppu:PP_4565)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EA1 at UniProt or InterPro

Protein Sequence (571 amino acids)

>PP_4565 Isocitrate dehydrogenase kinase/phosphatase (Pseudomonas putida KT2440)
MSQPWPAVEIARMILAGFDDYRDHFQRITLGARQRFEQARWQDIQQAAAARINLYEEKVA
EVNGWLRQAFAAEVLLDVEQWPLVKNAYIHLIDPRLDDELAETWYNSLFCSLFSHDLISD
GCMFIHTTRPSMRGRERAAQTRTYRLDGSLRNLLRAVFADYPFDMPYGDLEGDLARLEEQ
LRECLPDWVCKDPALAVELFVPVLYRNKGAYLVGRLYNSDEQWPLVIPLLHREGHGIEAD
ALITDEAEVSIIFSFTRSYFMADVPVPAEFVNFLKRILPGKHIAELYTSIGFYKQGKSEF
YRALINHLASSDDRFVMAPGVRGMVMSVFTLPGFNTVFKIIKDRFSPSKTVDRATVIDKY
RLVKSVDRVGRMADTQEFADFRFPRSKFEPDCLAELLEVAPSTVALEGDTVLIRHCWTER
RMTPLNLYLEQATEGQVLEALEDYGLAIKQLAAANIFPGDMLLKNFGVTRHGRVVFYDYD
EISFLTEVNFRHIPPPRYPEDEMSGEPWYSIGPHDVFPEEFPPFLFADMGQRRLFSRLHG
ELYDADYWKGLQAAIREGKVIDVFPYRRKAR