Protein Info for PP_4542 in Pseudomonas putida KT2440

Annotation: putative ABC transporter, ATP-binding protein/permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 610 transmembrane" amino acids 40 to 62 (23 residues), see Phobius details amino acids 84 to 105 (22 residues), see Phobius details amino acids 160 to 179 (20 residues), see Phobius details amino acids 185 to 204 (20 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details amino acids 297 to 322 (26 residues), see Phobius details PF00664: ABC_membrane" amino acids 42 to 304 (263 residues), 53.3 bits, see alignment E=3.4e-18 PF00005: ABC_tran" amino acids 379 to 532 (154 residues), 120.7 bits, see alignment E=7.4e-39

Best Hits

Swiss-Prot: 53% identical to Y1051_HAEIN: Uncharacterized ABC transporter ATP-binding protein HI_1051 (HI_1051) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K06147, ATP-binding cassette, subfamily B, bacterial (inferred from 100% identity to ppu:PP_4542)

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EC4 at UniProt or InterPro

Protein Sequence (610 amino acids)

>PP_4542 putative ABC transporter, ATP-binding protein/permease protein (Pseudomonas putida KT2440)
MLYRRFERLIDIFREAPSEAPPSTVWPFYLYYLRQVWPSFVALLVVGLVASLIEVAMFSY
LSRIIDLAQGTPNANFFSEHSGELIWMLVVILLLRPLFFGLHDLLVHQTINPGMTSLIRW
QNHTYVLKQSLNFFQSDFAGRIAQRIMQTGNSLRDSAVQAVDALWHVLIYAITSLVLFAE
ADWRLMLPLLAWIVSYIAALFYFVPRVKERSVVSSDARSKLMGRIVDGYTNIATLKLFAH
TDYEQQYAREAIREQTEKTQLASRVITSMDVVITTLNGLLVVATTGLALWLWSQSLITVG
AIALATGLVIRIVNMSGWIMWVVNGIFENIGMVQDGLQTIAQPVTVTDPPNAPALKVSRG
AVRFVDVDFHYGKATNVIEGLNLDIRPGEKIGLIGPSGAGKSTLVNLLLRLYDVQGGRIL
IDGQDIAKVSQASLRAQIGMITQDTSLLHRSIRENLLYGRPGASDAELHEAVRRARADEF
IPQLSDAQGRTGFDAHVGERGVKLSGGQRQRIAIARVLLKNAPILIMDEATSALDSEVEA
AIQESLETLMQGKTVIAIAHRLSTIARMDRLVVLDKGRIVESGSHSELLEQQGLYARLWH
HQTGGFVGVD