Protein Info for PP_4537 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 29 to 47 (19 residues), see Phobius details amino acids 59 to 79 (21 residues), see Phobius details amino acids 88 to 89 (2 residues), see Phobius details amino acids 109 to 129 (21 residues), see Phobius details amino acids 136 to 156 (21 residues), see Phobius details amino acids 174 to 193 (20 residues), see Phobius details amino acids 205 to 223 (19 residues), see Phobius details amino acids 230 to 254 (25 residues), see Phobius details amino acids 260 to 280 (21 residues), see Phobius details PF00892: EamA" amino acids 3 to 127 (125 residues), 37.7 bits, see alignment E=1.2e-13 amino acids 138 to 277 (140 residues), 38.9 bits, see alignment E=5e-14 TIGR00950: carboxylate/amino acid/amine transporter" amino acids 12 to 274 (263 residues), 251.1 bits, see alignment E=6.3e-79

Best Hits

Swiss-Prot: 72% identical to BIOP_PSEAE: Probable biotin transporter (PA3474) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_1354)

Predicted SEED Role

"MadN protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EC9 at UniProt or InterPro

Protein Sequence (291 amino acids)

>PP_4537 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MGYLIIVALIQAFSFSLIGEYLAGHVDSYFAVLARVLLAGLVFLPLTRWRQVEPRFMRSM
LLIGALQYGITYVCLYLSFRVLTVPEVLLFTILTPLHVTLIEDALNRRFNAWALVAALVA
VAGAAVIRFDSISGEFFIGFLLLQLANFTYAAGQVLYRHLVARHPSDLPHYARFGYFYLG
ALLVVLPAFLLFGNTQHLPSTDVQWLVLLFLGLCPTALGLYWWNKGACLVSGGTLAVMNN
LHVPVGLLLNLLIWNQHEPLGRLFIGGGIILASVWLSRLGGRAQAPLAGKA