Protein Info for PP_4512 in Pseudomonas putida KT2440

Annotation: putative permease of the drug/metabolite transporter (DMT) superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 34 to 56 (23 residues), see Phobius details amino acids 69 to 87 (19 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 123 to 141 (19 residues), see Phobius details amino acids 148 to 166 (19 residues), see Phobius details amino acids 177 to 196 (20 residues), see Phobius details amino acids 207 to 231 (25 residues), see Phobius details amino acids 237 to 256 (20 residues), see Phobius details amino acids 262 to 283 (22 residues), see Phobius details PF00892: EamA" amino acids 10 to 136 (127 residues), 53.7 bits, see alignment E=1.3e-18 amino acids 149 to 279 (131 residues), 64.1 bits, see alignment E=8.3e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4512)

Predicted SEED Role

"membrane protein, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EF3 at UniProt or InterPro

Protein Sequence (296 amino acids)

>PP_4512 putative permease of the drug/metabolite transporter (DMT) superfamily (Pseudomonas putida KT2440)
MNHRTALGALHIGALFFGLTGVFGKLATSASPAIIVFGRATFAVLALALFASLAGPGWQR
LSSQDARRLLLGGVLLAGHWVSFFIAVKVGGVAIATLGFASFPAFTVILESLLFRERIHR
NEAVLVVLVSIGLILVTPAFDLASEATGGLLWALLSGLLFSLLSLTNRAGSGRLPAVQAA
LWQNLVVGLCLLPFAAPGLADVAALDWLWIALLGIFCTGVAHSLFVASLAVIKARTAAVV
FGMEPVYGIAVAWAVFAETPTVRMLLGGALIIFAIVLSSRLAAEQPPRQRPLTEGA