Protein Info for PP_4457 in Pseudomonas putida KT2440

Annotation: opine oxidase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 210 to 229 (20 residues), see Phobius details PF01266: DAO" amino acids 6 to 347 (342 residues), 204.7 bits, see alignment E=1.5e-64

Best Hits

Swiss-Prot: 40% identical to OOXB_AGRT4: Opine oxidase subunit B (ooxB) from Agrobacterium tumefaciens (strain Ach5)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4457)

MetaCyc: 40% identical to octopine oxidase subunit B (Agrobacterium tumefaciens)
1.5.-.-

Predicted SEED Role

"D-nopaline/octopine oxidase subunit B @ Opine oxidase subunit B"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EK5 at UniProt or InterPro

Protein Sequence (375 amino acids)

>PP_4457 opine oxidase subunit B (Pseudomonas putida KT2440)
MTAQFDVIIIGGGMVGAAIGYGLAGGKRRILMLDGADTDYRAAKANFGLVWAHGKGMRDP
NYQRLSLNAAKAWPSFASQLEEESGISLDYEQKGGLNFCLSDDEFDARAKAMDAWNRQTP
ELAPSAHMLDRAELLRRFPTMKIGKAVVGASFGEFDGHANPLRLLAAMQKAFMARGGQLQ
NNRPVTDIRRLSGGGFEVQSGEYRAQCERIVIAAGLGCAALGPMVGLNVPIRPQRGQLLV
TERLAPLLPIPGSGLRQTAEGTVMIGVTQEEVGFDLATTTEGAARITRKALKILPDLAQA
KLVRQWSCLRIMTPDGNPVYARSLTHPGAEIATCHSGVTLASFHANEYAKAFDAKSIPAD
LEIFHYGRFDLQKTS