Protein Info for PP_4428 in Pseudomonas putida KT2440

Annotation: Amino acid ABC transporter, periplasmic amino acid-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR02995: ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB" amino acids 7 to 282 (276 residues), 478.4 bits, see alignment E=3e-148 PF00497: SBP_bac_3" amino acids 43 to 252 (210 residues), 150 bits, see alignment E=3.7e-48

Best Hits

KEGG orthology group: K02030, polar amino acid transport system substrate-binding protein (inferred from 100% identity to ppu:PP_4428)

Predicted SEED Role

"amino acid ABC transporter, periplasmic amino acid-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EM6 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PP_4428 Amino acid ABC transporter, periplasmic amino acid-binding protein (Pseudomonas putida KT2440)
MNLVTSLSAVAQRLTLACAIASIAVSAHAATTLDSLKEQGSVRIGYANETPFAYTGTDGK
VTGESPEIATAIFKQLGIKDVNGVLTEWGSLIPGLRAGRFDAIAAGMYITPARCKQVLFT
DPHYQLPDALLVPKGNPKELFSYEDVAKNPEVKLAIMAGTVELQYARDSGVKDEQILQVP
DTTAQLQAVRAKRADAAVGTQLTMKGLAAKGGDSVEAMTDFKDDPSHIGYGALAFRPEDK
ELRDQVNAQLKKWLGSEEHLKTVAPFGFDKSNLTTKTAAELCGQ