Protein Info for PP_4400 in Pseudomonas putida KT2440

Annotation: Bkd operon transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 161 PF13404: HTH_AsnC-type" amino acids 4 to 45 (42 residues), 54.9 bits, see alignment E=9e-19 PF13412: HTH_24" amino acids 4 to 51 (48 residues), 67 bits, see alignment E=1.2e-22 PF01037: AsnC_trans_reg" amino acids 70 to 143 (74 residues), 85.2 bits, see alignment E=3.6e-28

Best Hits

Swiss-Prot: 99% identical to BKDR_PSEPU: Bkd operon transcriptional regulator (bkdR) from Pseudomonas putida

KEGG orthology group: K03719, Lrp/AsnC family transcriptional regulator, leucine-responsive regulatory protein (inferred from 98% identity to ppw:PputW619_3741)

Predicted SEED Role

"Transcriptional regulator BkdR of isoleucine and valine catabolism operon" in subsystem Isoleucine degradation or Valine degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EQ3 at UniProt or InterPro

Protein Sequence (161 amino acids)

>PP_4400 Bkd operon transcriptional regulator (Pseudomonas putida KT2440)
MRKLDRTDIGILNSLQENARITNAELARSVNLSPTPCFNRVRAMEELGVIRQQVTLLSPE
VLGLDVNVFIHVSLEKQVEQSLHRFEEEIAERPEVMECYLMTGDPDYLLRVLLPSIQALE
RFLDYLTRLPGVANIRSSFALKQVRYKTALPLPANGMTLRE