Protein Info for PP_4392 in Pseudomonas putida KT2440

Annotation: chemotaxis protein methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 PF03705: CheR_N" amino acids 7 to 58 (52 residues), 48 bits, see alignment 1.2e-16 PF01739: CheR" amino acids 73 to 267 (195 residues), 202.9 bits, see alignment E=5.2e-64

Best Hits

Swiss-Prot: 100% identical to CHER2_PSEPK: Chemotaxis protein methyltransferase Cher2 (cheR2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00575, chemotaxis protein methyltransferase CheR [EC: 2.1.1.80] (inferred from 99% identity to ppg:PputGB1_3953)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ER1 at UniProt or InterPro

Protein Sequence (275 amino acids)

>PP_4392 chemotaxis protein methyltransferase (Pseudomonas putida KT2440)
MSTGNLDFEQFRVFLEKACGILLGENKQYLVSSRLNKLMEQQGIKSLGELVQRIQAQPRG
GLREQVVDAMTTNETLWFRDTYPFEVLKNKVIPEFIRNNPGQRLRMWSAACSSGQEPYSI
SMAIDEFERSNLGQLKMGAQIVATDLSGTMLTNCKTGEYDSLAIARGLSQERLQRYFDPK
GPGRWAVKPAIRSRVEFRSFNLLDSYASLGKFDIVFCRNVLIYFSAQVKKDILLRIHSTL
KPGGYLFLGASEALNGLPDHYQMVQCSPGIIYQAK