Protein Info for PP_4381 in Pseudomonas putida KT2440

Annotation: Flagellar hook-associated protein FlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR02492: flagellar hook-associated protein FlgK" amino acids 4 to 349 (346 residues), 238.2 bits, see alignment E=1.1e-74 PF00460: Flg_bb_rod" amino acids 7 to 34 (28 residues), 30.7 bits, see alignment (E = 3.6e-11) PF21158: flgK_1st_1" amino acids 338 to 418 (81 residues), 26.3 bits, see alignment E=9.2e-10 PF06429: Flg_bbr_C" amino acids 629 to 678 (50 residues), 27.5 bits, see alignment 4e-10

Best Hits

KEGG orthology group: K02396, flagellar hook-associated protein 1 FlgK (inferred from 100% identity to ppu:PP_4381)

Predicted SEED Role

"Flagellar hook-associated protein FlgK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ES2 at UniProt or InterPro

Protein Sequence (680 amino acids)

>PP_4381 Flagellar hook-associated protein FlgK (Pseudomonas putida KT2440)
MSSLISIGLSGLSASQAALSVTSNNIANAATSGYSRQQTVQAAGPSHNIGAGFLGTGTTL
ADVRRIYSSYLDNQLQTATSLQADSVTFQDQITSVDKLLADRDTGISSVLTAFFSALQTA
AAKPGDVASRQLLLTQAQTLSNRFNAVSTQLTQQNATINSQLDTMAGQVNKLTANIAEYN
KQITAASASGNTPNSLLDARSEAVRQLNELVGVTVQERDGNYDVYLGSGQSLVTGTKANT
LSVEPGVADKSQVALRINYDSFSSDVTSVVSGGAIGGLLRYRQDVLTPSMNELGRVALVV
ADSINSQLGQGLDANGQFGSSLFSSINSATAIAQRSLASSNNSAGSGNLDVTIANSGALT
TYDYEVKFTSASQYSVRRSDGTDMGSFDLNADPAPVIDGFSLALKGGGLAAGDSFKVIPT
RAAAGSITTTLTDANKLAFAGPISAAAGSGNSGTGTITQPTLGESLDIYGGADTALIQQS
IRDSMPVRVVFDAASGGSQGYKLFDAKGTQIGTGSVVPGQDNKLSIAVPMLDAAGDPILD
GSGNPRTFAVETTIGGSPAANDSFTLSFNADGKADNRNANALLDLQTKSTVGTNSGTGTS
FTSAYASLVERVGAKASQATIDTTATQAVLKSATESRSAVSGVNLDDEAASLVKFQHYYT
ASSQIIKAAQETFSTLINAL