Protein Info for PP_4372 in Pseudomonas putida KT2440

Annotation: Sensory box histidine kinase FleS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 PF13188: PAS_8" amino acids 78 to 134 (57 residues), 37.6 bits, see alignment 2.9e-13 PF00989: PAS" amino acids 79 to 115 (37 residues), 27 bits, see alignment 7.8e-10 PF00512: HisKA" amino acids 185 to 250 (66 residues), 43.9 bits, see alignment E=4.1e-15 PF02518: HATPase_c" amino acids 294 to 396 (103 residues), 80.5 bits, see alignment E=2.5e-26

Best Hits

KEGG orthology group: K10942, two-component system, sensor histidine kinase FlrB [EC: 2.7.13.3] (inferred from 100% identity to ppf:Pput_1495)

Predicted SEED Role

"Flagellar sensor histidine kinase FleS" in subsystem Flagellum

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ET1 at UniProt or InterPro

Protein Sequence (405 amino acids)

>PP_4372 Sensory box histidine kinase FleS (Pseudomonas putida KT2440)
MPQAAHVSRAPDQQGQTPVAQDSGQGIAQAFALFNQVSSQLSQSYSLLEARVSELKGELA
VVSAQRIDELSEKERLANRLQNLLDLLPGGVIVIDGQGLVREANPAACELLGEPLIGQLW
RQVIARSFAPRKDDGHEVSLRDGRRLSIATRSLDAEPGQLVLLNDLTETRRLQDQLARHE
RLSSLGRMVASLAHQIRTPLSAAMLYAGHLADNEQALSAQTRQRFAATLKERLHELEHQV
RDMLVFARGELPLGDRVTPKALFQALQQAAQAHVQGHAVRWQCDSHLGELLCNRDTLVGA
LLNLIENALQASDGPARLKVHLYRRERSLHLCVSDAGSGIDAGLLGRLGEPFLTTKATGT
GLGLAVVKAVVRAHQGTINLRSKVGRGTCVRVELPLIDGQLAEGV