Protein Info for PP_4299 in Pseudomonas putida KT2440

Annotation: tartronate semialdehyde reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 TIGR01505: 2-hydroxy-3-oxopropionate reductase" amino acids 3 to 291 (289 residues), 404.6 bits, see alignment E=1.2e-125 PF03807: F420_oxidored" amino acids 3 to 94 (92 residues), 34.7 bits, see alignment E=4.6e-12 PF03446: NAD_binding_2" amino acids 3 to 162 (160 residues), 182.5 bits, see alignment E=1.2e-57 PF07991: IlvN" amino acids 3 to 71 (69 residues), 26.8 bits, see alignment E=7e-10 PF14833: NAD_binding_11" amino acids 165 to 283 (119 residues), 135.9 bits, see alignment E=1.7e-43

Best Hits

Swiss-Prot: 64% identical to GLXR_ECOLI: 2-hydroxy-3-oxopropionate reductase (glxR) from Escherichia coli (strain K12)

KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 99% identity to ppf:Pput_1570)

MetaCyc: 64% identical to tartronate semialdehyde reductase 2 (Escherichia coli K-12 substr. MG1655)
1.1.1.60,4.1.1.M27,4.1.1.73 [EC: 1.1.1.60, 4.1.1.73, 4.1.1.M27]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.60 or 4.1.1.73 or 4.1.1.M27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F01 at UniProt or InterPro

Protein Sequence (297 amino acids)

>PP_4299 tartronate semialdehyde reductase (Pseudomonas putida KT2440)
MAKIGFIGTGIMGKPMAQNLQKAGHSLFISTHHDAAPADLIAAGAVALANPKEVAQEAEF
IIVMVPDTPQVESVLFGENGVAEGVGPNKVVIDMSSISPTATKAFAEKIKATGAAYLDAP
VSGGEVGAKAATLSIMVGGCPNAFERTLPLFEAMGKNITRVGGNGDGQTAKVANQIIVAL
NIQAVAEALLFAAKNGADPAKVREALMGGFASSKILEVHAERMIKGTFDPGFRINLHQKD
LNLALQGAKELGINLPNTSNAQQVFNTCQALGGGNWDHSALIKGLEHMANFSIRDDK