Protein Info for PP_4282 in Pseudomonas putida KT2440

Annotation: Aquaporin Z

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 38 to 61 (24 residues), see Phobius details amino acids 82 to 104 (23 residues), see Phobius details amino acids 129 to 150 (22 residues), see Phobius details amino acids 160 to 180 (21 residues), see Phobius details amino acids 205 to 224 (20 residues), see Phobius details PF00230: MIP" amino acids 5 to 223 (219 residues), 174.3 bits, see alignment E=1.7e-55 TIGR00861: MIP family channel proteins" amino acids 9 to 223 (215 residues), 201.8 bits, see alignment E=6.4e-64

Best Hits

Swiss-Prot: 100% identical to AQPZ_PSEPK: Aquaporin Z (aqpZ) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K06188, aquaporin Z (inferred from 99% identity to ppg:PputGB1_3846)

MetaCyc: 70% identical to water channel AqpZ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-145

Predicted SEED Role

"Aquaporin Z"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F17 at UniProt or InterPro

Protein Sequence (230 amino acids)

>PP_4282 Aquaporin Z (Pseudomonas putida KT2440)
MSLGKRMGAELIGTFWLVLGGCGSAVLAASSPLGIGVLGVAFAFGLTVLTMAFAIGHISG
CHLNPAVSFGLVVGGRFPAKELLPYVIAQVIGAILAAGVIYLIASGKAGFELSAGLASNG
YADHSPGGYTLGAGFVSEVVMTAMFLVVIMGATDARAPAGFAPIAIGLALTLIHLISIPV
TNTSVNPARSTGPALFVGGWALQQLWLFWVAPLIGAAIGGALYRGLAKEP