Protein Info for PP_4164 in Pseudomonas putida KT2440

Annotation: Alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 PF00561: Abhydrolase_1" amino acids 29 to 144 (116 residues), 83.2 bits, see alignment E=4.7e-27 PF12697: Abhydrolase_6" amino acids 30 to 271 (242 residues), 54.7 bits, see alignment E=4.6e-18 PF12146: Hydrolase_4" amino acids 30 to 125 (96 residues), 36.5 bits, see alignment E=6.8e-13 PF00975: Thioesterase" amino acids 34 to 150 (117 residues), 34.7 bits, see alignment E=4.4e-12

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_1703)

Predicted SEED Role

"hydrolase, alpha/beta fold family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FD4 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PP_4164 Alpha/beta hydrolase (Pseudomonas putida KT2440)
MSAHVEEIRLSLGHIELAAHLFGPEDGLPVIALHGWLDNANSFARLAPQLKGLRLVALDL
AGHGYSEHRPLGAGYALADYAHDVLRVAEQLGWPRFALLGHSLGAIISVQLAGALPDRVS
HLALIDGVIPPTGAEQDAGERLGMALQAQLRLDGKRKSVYATLEAGVQARMKGMVAVSRE
AAELLAQRGLMPVPGGYSWRSDSRLTLPSPVRLSEAQAMAYVRRVSCPALLVVAADGMLA
RHTGLLEQLPFEQVTLPGGHHLHLNDAQGAALVADCINRFFGFA