Protein Info for PP_4147 in Pseudomonas putida KT2440

Annotation: microcin C transporter - periplasmic binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 611 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00496: SBP_bac_5" amino acids 106 to 510 (405 residues), 234.1 bits, see alignment E=1.5e-73

Best Hits

Swiss-Prot: 43% identical to Y3691_BRUME: Putative binding protein BMEII0691 (BMEII0691) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K13893, microcin C transport system substrate-binding protein (inferred from 100% identity to ppu:PP_4147)

Predicted SEED Role

"ABC transporter, periplasmic substrate-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FF0 at UniProt or InterPro

Protein Sequence (611 amino acids)

>PP_4147 microcin C transporter - periplasmic binding protein (Pseudomonas putida KT2440)
MTPTHRLRRLAGSLLLACLSLPALAAPQHALTLYDEPPKYPADFKHFDYVNPDAPKGGTF
RQSSFGGFDSLNPFINKGVAAENIGIIYDTLMRQSQDEPFTEYGLVAGKIEKAPDNSWVR
FYLRPEARFHDGHPMRADDVVFTFNALIKDGAPLYRQYYADVAEVVAEEPLKVLFKFKHK
NNRELPLILGQLPVLPKHWYENREFNRGNLEIPLGSGPYKVAKVKAGRSVRYERVKDYWA
KDLPINRGFYNFDAMTFDAYRDTTVALEALKAGAFDYGLEVSAKNWATAYNVPAVREGRL
IKEELPNGNPTGMQGFIFNIRRPVFQDVRVRQALSLLLDYEWTNKQLFNGAYTRTGSYFE
NSEMAARGLPSPNELKILEPLRGKVPEQVFTEVFHNPVSDGSGMIREQQRQAYKLLQEAG
WKIVDDKMVDAQGKPASIEFLLAQTEFERILLPFKRNLADLGIELNIRRVDVSQYITRLR
SRDYDMIVGGYPQSNSPGNEQREFWSSAAADNPGSRNFIGLRDPAIDQLVDQLINADSRQ
SLIDHCRALDRVLLWGYYVIPNWHIKTWRVAYWNHIGHPNVSPKYDIGIDTWWIKPDVTP
AVSEAPADEAN