Protein Info for PP_4130 in Pseudomonas putida KT2440

Annotation: NADH:ubiquinone oxidoreductase, membrane subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 30 to 48 (19 residues), see Phobius details amino acids 82 to 105 (24 residues), see Phobius details amino acids 115 to 135 (21 residues), see Phobius details amino acids 141 to 159 (19 residues), see Phobius details amino acids 175 to 198 (24 residues), see Phobius details amino acids 219 to 240 (22 residues), see Phobius details amino acids 259 to 278 (20 residues), see Phobius details amino acids 288 to 309 (22 residues), see Phobius details amino acids 316 to 334 (19 residues), see Phobius details amino acids 340 to 362 (23 residues), see Phobius details amino acids 383 to 401 (19 residues), see Phobius details amino acids 420 to 442 (23 residues), see Phobius details amino acids 463 to 481 (19 residues), see Phobius details TIGR01972: proton-translocating NADH-quinone oxidoreductase, chain M" amino acids 3 to 497 (495 residues), 571 bits, see alignment E=1e-175 PF00361: Proton_antipo_M" amino acids 134 to 432 (299 residues), 236.7 bits, see alignment E=1.6e-74

Best Hits

Swiss-Prot: 81% identical to NUOM_PSEAE: NADH-quinone oxidoreductase subunit M (nuoM) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00342, NADH dehydrogenase I subunit M [EC: 1.6.5.3] (inferred from 100% identity to ppf:Pput_1735)

MetaCyc: 100% identical to NADH-quinone oxidoreductase subunit M (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FG6 at UniProt or InterPro

Protein Sequence (510 amino acids)

>PP_4130 NADH:ubiquinone oxidoreductase, membrane subunit M (Pseudomonas putida KT2440)
MILPWLILIPFIGGFLCWLGERFGATLPRWIALLTMSLLLGIGLWLWGTGDYTLAPAPGA
EPAWALEYKVEWIKRFGISIHLALDGLSLLMILLTGLLGVLSVLCSWKEIQRHVGFFHLN
LMWILGGVVGVFLALDLFLFFFFWEMMLVPMYFLIALWGHSSADGKKTRIYAATKFFIFT
QASGLIMLVAILGLVLVNYNTTGVLTFNYSDLLKAELPAGIEYVLMLGFFIAFAVKLPVV
PFHSWLPDAHAQAPTAGSVDLAGILLKTAAYGLLRFALPLFPNASAEFAPIAMTLGLIGI
FYGAFLAFAQTDIKRLIAFSSVSHMGFVLIGIYSGSQQALQGAVIQMLAHGLSAAALFIL
SGQLYERLHTRDMRQMGGLWHRIAYLPAISLFFAAASLGLPGTGNFVGEFLILIGSFVHV
PWITVIATTGLVFGSVYSLIMIHRAYFGPAKTDTVLAGMDGRELIMVLGLAVLLILLGVY
PQPFLDTSAATMSGVQQWLGSAFTQLASAR