Protein Info for PP_4129 in Pseudomonas putida KT2440

Annotation: NADH:ubiquinone oxidoreductase, membrane subunit L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 31 to 50 (20 residues), see Phobius details amino acids 79 to 82 (4 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 117 to 134 (18 residues), see Phobius details amino acids 140 to 160 (21 residues), see Phobius details amino acids 172 to 195 (24 residues), see Phobius details amino acids 215 to 233 (19 residues), see Phobius details amino acids 250 to 269 (20 residues), see Phobius details amino acids 281 to 302 (22 residues), see Phobius details amino acids 309 to 329 (21 residues), see Phobius details amino acids 334 to 357 (24 residues), see Phobius details amino acids 377 to 399 (23 residues), see Phobius details amino acids 416 to 436 (21 residues), see Phobius details amino acids 456 to 475 (20 residues), see Phobius details amino acids 497 to 517 (21 residues), see Phobius details amino acids 529 to 548 (20 residues), see Phobius details amino acids 596 to 616 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 2 to 612 (611 residues), 776 bits, see alignment E=2e-237 PF00662: Proton_antipo_N" amino acids 69 to 119 (51 residues), 70.1 bits, see alignment 1.2e-23 PF00361: Proton_antipo_M" amino acids 136 to 427 (292 residues), 289.1 bits, see alignment E=3.6e-90

Best Hits

Swiss-Prot: 83% identical to NUOL_PSEAE: NADH-quinone oxidoreductase subunit L (nuoL) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to ppu:PP_4129)

MetaCyc: 100% identical to NADH-quinone oxidoreductase subunit L (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FG7 at UniProt or InterPro

Protein Sequence (617 amino acids)

>PP_4129 NADH:ubiquinone oxidoreductase, membrane subunit L (Pseudomonas putida KT2440)
MNLLFLTFVFPLIGFLLLSFSRGRFSENLSALIGVGSVGLSAATAAYVIWQFNVAPPEGG
AYSQLLWQWMSVDGFAPNFTLYLDGLSVTMLGVVTGVGFLIHLFASWYMRGEAGYSRFFS
YTNLFIASMLFLILGDNLLFIYFGWEGVGLCSYLLIGFYYSNRNNGNAALKAFIVTRIGD
VFMAIGLFILFAQLGTLNVQELLVLAPQKFQAGDTWMVLATLMLLGGAVGKSAQLPLQTW
LADAMAGPTPVSALIHAATMVTAGVYLIARTNGLFLLAPDILHLVGVVGGLTLVLAGFAA
LVQTDIKRILAYSTMSQIGYMFLALGVGAWDAAIFHLMTHAFFKALLFLASGAVIVACHH
EQNIFKMGGLWKKLPLAYASFVVGGAALAALPIVTVGFYSKDEILWEAFASGNTGLLYAG
LVGAFMTSLYTFRLIFIAFHGEAKTEAHAGHGISHWLPLGVLIVLSTFVGAWITPPLAGV
LPESAGHAGGEAKHALEITSGAIAIAGILLAALLFLGKRRFVSAVANSGIGRVLSAWWFA
AWGFDWIYDKLFVKPYLLISHILRKDPVDRTIGLIPRMARGGHVAMSKTETGQLRWYTAS
IAVGAVLVLGAVVVAAV