Protein Info for PP_4124 in Pseudomonas putida KT2440

Annotation: NADH-quinone oxidoreductase subunit G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 904 PF13510: Fer2_4" amino acids 3 to 77 (75 residues), 41.3 bits, see alignment E=3.2e-14 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 651 (648 residues), 696.3 bits, see alignment E=2.3e-213 PF00111: Fer2" amino acids 5 to 71 (67 residues), 30 bits, see alignment 9.8e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 127 (40 residues), 56.6 bits, see alignment (E = 3.6e-19) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 49.6 bits, see alignment 7.6e-17 PF00384: Molybdopterin" amino acids 361 to 634 (274 residues), 50.8 bits, see alignment E=3.4e-17

Best Hits

Swiss-Prot: 100% identical to NUOG_PSEPK: NADH-quinone oxidoreductase subunit G (nuoG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 78% identity to avn:Avin_28490)

MetaCyc: 65% identical to NADH:quinone oxidoreductase subunit G (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FH2 at UniProt or InterPro

Protein Sequence (904 amino acids)

>PP_4124 NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
MATIHVDGKALEVNGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYTDENDTR
GRIVMSCMTPASDGTWISIDDEESKAFRASVVEWLMTNHPHDCPVCEEGGHCHLQDMTVM
TGHNERRYRFTKRTHQNQDLGPFIAHEMNRCIACYRCVRYYKDYAGGTDLGVYGAHDNVY
FGRVEDGVLESEFSGNLTEVCPTGVFTDKTHSERYNRKWDMQFAPSICHGCSSGCNISPG
ERYGELRRIENRFNGSVNQYFLCDRGRFGYGYVNRKDRPRQPQLADGTKLGLDAALDKAA
DLLRGRTIVGIGSPRASLESNYGLRELVGAEYFYSGMEAGELARVRLALNVLNNSPLPVP
TLRDIEDHDAVFVLGEDLTQTAARVALAVRQATKGKAEAMAEAMKVQPWLDAAVKNIGQH
ALYPLFIASLAETKLDDVAEECVHAAPADLARIGFAVAHAIDPSAPAVAGLDDEAQALAQ
RIADALVAAKRPLVVAGTSLADPALIEAAANIAKALKLREKNGSLSLVVPEANSLGLAML
GGESVDAALDAVISGKADAIVVLENDLYARVPAAKVDAALAAAKVVIVADHSKTATVDRA
HLVLPAASFAEGDGTLVSQEGRAQRFFQVFDPQYLDSSIQIHEGWRWMHALRATLLNKPV
DWTQLDHVTSACAEAAPQLAGIVNAAPSAAFRIKGMKLAREPLRYSGRTAMRANISVHEP
RTPQDKDTAFAFSMEGYSGSAEPRQQVPFAWSPGWNSPQAWNKFQDEVGGHLRAGDPGVR
LIESQGDRLNWFNAIPGAFNPARGIWTAVPFFHLFGSEESSSRAAPVQERIPAAYVALAK
SEADRLGVNDGALLSLNVAGVALRLPLRINEELGAGLVALPKGLAGIPPAIFGASVEGLQ
EAAQ