Protein Info for PP_4123 in Pseudomonas putida KT2440

Annotation: NADH-quinone oxidoreductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01959: NADH oxidoreductase (quinone), F subunit
  • amino acids 21 to 433 (413 residues), 662 bits, E=1.1e-203
• PF01512: Complex1_51K
  • amino acids 62 to 234 (173 residues), 176.4 bits, E=3.7e-56
• PF10589: NADH_4Fe-4S
  • amino acids 348 to 431 (84 residues), 88 bits, E=3.1e-29

Best Hits

Swiss-Prot: 89% identical to NUOF_PSEAE: NADH-quinone oxidoreductase subunit F (nuoF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 100% identity to ppf:Pput_1742)

MetaCyc: 100% identical to NADH-quinone oxidoreductase subunit F (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• Fe(II) oxidation (3/6 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See Q88FH3 at UniProt or InterPro

Protein Sequence (453 amino acids)

>PP_4123 NADH-quinone oxidoreductase subunit F (Pseudomonas putida KT2440)
MTITSFGPANRIARSAETHPLTWRLRDDGEPVWLAEYESKNGYAAARKALAQMSADDIVQ
SVKDSGLKGRGGAGFPTGVKWGLMPKDESMNIRYLLCNADEMEPNTWKDRMLMEQQPHLL
VEGMLISARALKAYRGYIFLRGEYTTAAKNLNRAIDEAKAAGLLGKNILGSGFDFELFVH
TGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIVANG
NDWYKSLAREGSEDHGTKLMGFSGKVKNPGLWELPFGVTARELFEDYAGGMRDGFKLKCW
QPGGAGTGFLLPEHLDAQMYAGGIAKVGTRMGTGLAMAVDDSINMVSLLRNMEEFFARES
CGWCTPCRDGLPWSVKMLRALENGQGRAEDIETLLGLVNFLGPGRTFCAHAPGAVEPLGS
AIKYFRSEFEAGVAPESAATLRPDLAKPIVVGA