Protein Info for PP_4108 in Pseudomonas putida KT2440

Updated annotation (from data): 2-aminoadipate transaminase (EC 2.6.1.39)
Rationale: Specifically important in carbon source D-Lysine. D-lysine. This is part of the pathway from D-lysine to 2-oxoglutarate. For detailed rationale and biochemical evidence, see https://doi.org/10.1101/450254
Original annotation: putative 4-aminobutyrate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00202: Aminotran_3
  • amino acids 15 to 412 (398 residues), 369.9 bits, E=7.1e-115

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4108)

MetaCyc: 100% identical to 4-aminobutyrate aminotransferase (Pseudomonas putida KT2440)
2-aminoadipate transaminase. [EC: 2.6.1.39]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

• L-lysine degradation V (9/9 steps found)
• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt II (3/4 steps found)
• GABA shunt I (2/4 steps found)
• L-lysine degradation II (L-pipecolate pathway) (5/9 steps found)
• L-lysine degradation XI (2/5 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• L-lysine biosynthesis IV (2/9 steps found)
• L-lysine biosynthesis V (2/10 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)
• superpathway of L-lysine degradation (23/43 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Butanoate metabolism
• Glutamate metabolism
• Lysine biosynthesis
• Lysine degradation
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19

Use Curated BLAST to search for 2.6.1.19 or 2.6.1.39

Sequence Analysis Tools

See Q88FI7 at UniProt or InterPro

Protein Sequence (416 amino acids)

>PP_4108 2-aminoadipate transaminase (EC 2.6.1.39) (Pseudomonas putida KT2440)
MNQESISQSIAIVHPITLSHGRNAEVWDTDGKRYIDFVGGIGVLNLGHCNPAVVEAIQAQ
ATRLTHYAFNAAPHGPYLALMEQLSQFVPVSYPLAGMLTNSGAEAAENALKVARGATGKR
AIIAFDGGFHGRTLATLNLNGKVAPYKQRVGELPGPVYHLPYPSADTGVTCEQALKAMDR
LFSVELAVEDVAAFIFEPVQGEGGFLALDPAFAQALRRFCDERGILIIIDEIQSGFGRTG
QRFAFPRLGIEPDLLLLAKSIAGGMPLGAVVGRKELMAALPKGGLGGTYSGNPISCAAAL
ASLAQMTDENLATWGERQEQAIVSRYERWKASGLSPYIGRLTGVGAMRGIEFANADGSPA
PAQLAKVMEAARARGLLLMPSGKARHIIRLLAPLTIEAEVLEEGLDILEQCLAELN