Protein Info for PP_4073 in Pseudomonas putida KT2440

Annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR03360: type VI secretion-associated protein, VC_A0118 family" amino acids 25 to 210 (186 residues), 249.8 bits, see alignment E=8.6e-79 PF11319: VasI" amino acids 27 to 210 (184 residues), 216.3 bits, see alignment E=1.4e-68

Best Hits

KEGG orthology group: K11909, type VI secretion system protein VasI (inferred from 100% identity to ppu:PP_4073)

Predicted SEED Role

"Type VI secretion protein VasI"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FL6 at UniProt or InterPro

Protein Sequence (210 amino acids)

>PP_4073 conserved exported protein of unknown function (Pseudomonas putida KT2440)
MIIRYTGGVGLSLLFLLMPALTRASPDCPRIVSNIERLACFDQAAGTPGFTPQRQWSAQE
LEAPTVRRVLIHETGRAPEDLRFRLRSEEGGLLISAPAIASVAPHPYLIISCVQNISRLQ
LVTAQPVDASRVQVRLRGERGATVPTPWQVMENGQVLDAGRGLPGIEQIKQLIGAHRIHV
ESDNPAVDGLVFDAQGLDPLIDEARKTCRW