Protein Info for PP_4067 in Pseudomonas putida KT2440

Annotation: methylcrotonyl-CoA carboxylase biotin-containing subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00289: Biotin_carb_N" amino acids 7 to 114 (108 residues), 140.1 bits, see alignment E=1.8e-44 PF02786: CPSase_L_D2" amino acids 120 to 326 (207 residues), 269 bits, see alignment E=1.2e-83 PF02222: ATP-grasp" amino acids 141 to 296 (156 residues), 41.4 bits, see alignment E=5.5e-14 PF07478: Dala_Dala_lig_C" amino acids 144 to 295 (152 residues), 50 bits, see alignment E=1.2e-16 PF02785: Biotin_carb_C" amino acids 340 to 447 (108 residues), 124.8 bits, see alignment E=7.6e-40 PF21139: MCC_alpha_BT" amino acids 463 to 564 (102 residues), 117.9 bits, see alignment E=9.3e-38 PF00364: Biotin_lipoyl" amino acids 584 to 648 (65 residues), 63.8 bits, see alignment E=4.7e-21

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 100% identity to ppu:PP_4067)

MetaCyc: 77% identical to methylcrotonyl-CoA carboxylase alpha-subunit (Pseudomonas aeruginosa PAO1)
Methylcrotonoyl-CoA carboxylase. [EC: 6.4.1.4]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FM2 at UniProt or InterPro

Protein Sequence (650 amino acids)

>PP_4067 methylcrotonyl-CoA carboxylase biotin-containing subunit alpha (Pseudomonas putida KT2440)
MSRPALTTLLVANRGEIACRVMRTAKAMGLTTVAVHSATDRDARHSREADIRVDLGGTKA
AESYLLVDKLLAAAKASGAQAIHPGYGFLSENAGFARAIEQAGLIFLGPPASAIDAMGSK
SAAKALMEAAGVPLVPGYHGEAQDLDTFRAAAERIGYPVLLKASAGGGGKGMKVVEEESQ
LADALASAQREAQSSFGDARMLVEKYVLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQ
KVVEEAPAPGLSPELRRAMGEAAVRAAQAIGYVGAGTVEFLLDARGEFFFMEMNTRLQVE
HPVTEAITGLDLVAWQIRVACGEALPITQEQVPLIGHAIEVRLYAEDPANDFLPATGKLA
LYRESAPGEGRRVDSGVSEGDVVSPFYDPMLGKLIAWGEDREQARLRLLAMLDEFAIGGL
KTNIAFLRRILAHPAFAAAELDTGFIPRHQDVLLPAPRALPAAFWEAAAEAWLQGQAGHQ
REDDRHSPWSGSNGLRLGLPARSSLHLVSAGQDQAVALERSAASTWQLQGEQLVHDQNGV
RRQHLAIRRGGTLYLHWEGEMHAIEAFDPIAEAEASHSHQGGLGAPMNGSIVRVLVEPGQ
VVEAGTALLVLEAMKMEHSIRAPHAGTVKALFCLEGDMVSEGTVLVELVE