Protein Info for PP_4058 in Pseudomonas putida KT2440

Annotation: 1,4-alpha-glucan branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 113 to 734 (622 residues), 937 bits, see alignment E=2.4e-286 PF02922: CBM_48" amino acids 129 to 211 (83 residues), 80.4 bits, see alignment E=1.5e-26 PF00128: Alpha-amylase" amino acids 282 to 356 (75 residues), 34.3 bits, see alignment E=3e-12 PF02806: Alpha-amylase_C" amino acids 638 to 735 (98 residues), 84.8 bits, see alignment E=6.6e-28

Best Hits

Swiss-Prot: 100% identical to GLGB_PSEPK: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to ppu:PP_4058)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FN1 at UniProt or InterPro

Protein Sequence (736 amino acids)

>PP_4058 1,4-alpha-glucan branching enzyme (Pseudomonas putida KT2440)
MNVTTRENGGLRQRDLDALARAEHADPFAVLGPHGDGAGGQVVRAFLPNALKVRIQARDD
GRVLAEMEQGSLPGLFSAHLDKAQPYQLHIVWAGGEQVTEDPYSFGPQLGDMDLHLFAEG
NHRDLSGRFGAQPTQVDGIDGVCFSVWAPNARRVSVVGDFNNWDGRRHPMRLRHGAGVWE
LFIPRLGVGETYKFEVLGKDGILPLKADPLARATELPPSTASKVAGELSHAWQDHDWMAQ
RAQRHAYNAPLSIYELHPGSWRCELDEAGEIGRFYNWRELAERLVPYVQELGFTHIELLP
IMEHPFGGSWGYQPLSMFAPTSRYGSAEDFAAFIDACHQGGIGVLLDWVPAHFPTDEHGL
ARFDGTALYEYDNPLEGYHQDWNTLIYNLGRNEVRGFMMASALHWLKHFHIDGLRVDAVA
SMLYRDYSRKAGEWVPNRHGGRENLEAIDFIRHLNGVAAHEAPGALIIAEESTAWPGVSQ
PTQQGGLGFAYKWNMGWMHDTLHYIQNDPVHRTYHHNEMSFGLIYAYSEHFILPISHDEV
VHGKHSLIDKMPGDRWQKFANLRAYLTFMWAHPGKKLLFMGCEFGQWREWNHDAELDWYL
LQYPEHQGVQRLVGDLNRLYREEPALHEQDCQPQGFQWLIGDDAQNSVYAWLRWSSSGEP
VLVVANFTPVPREGYRIGVPFGERWQELLNSDAELYAGSNVGNLGAVASDEVASHGQPLS
LALNLPPLGVLIMKPA