Protein Info for PP_4053 in Pseudomonas putida KT2440

Annotation: maltooligosyl trehalose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 924 TIGR02401: malto-oligosyltrehalose synthase" amino acids 5 to 921 (917 residues), 927 bits, see alignment E=5.6e-283 PF00128: Alpha-amylase" amino acids 26 to 123 (98 residues), 49.8 bits, see alignment E=1.6e-17

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 100% identity to ppu:PP_4053)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FN6 at UniProt or InterPro

Protein Sequence (924 amino acids)

>PP_4053 maltooligosyl trehalose synthase (Pseudomonas putida KT2440)
MKPLTATLRLQFHSDFTLDHAVPLVPYFAQLGISHLYASPILKARAGSRHGYDVVDPTCV
NPELGGEAALERLVAALRQHGMGLILDTVSNHMAVGGADNPWWQSLLAWGRRSPYAEFFD
IQWHSSDPLLAGQLLLPFLGSDYGVALRNGEIPLQFDKQQGLLQVAHYAHRFPICPVDYG
WILALSPEPALKVLAERFTALGDSATPLADSLPLQAELARLVREGADLESALVAFDSRSE
AGFKRLHLLLERQTYRLASWRTAADDINWRRFFDINELGGLRVERAVVFEATHAKLFELI
ERGLVDGLRIDHIDGLADPRGYCRKLRRRVDSLLARRPLNAGLEHFPLYVEKILGADEHL
HRDWLTDGTTGYEFMNQVSLLQHDPAGEAPLSELWSNVSERPDFPEEVRQARHLVLNASL
AGDCESVAQALLQVARNDLMTRDLTLGAIRRALQALVAHYPVYRTYFNACGRPAQDETFF
QQALTNARQDLAEADWPLLDQLERWLGGQAWRHLPPGRARKQLRHACVRFQQLTAPSAAK
AVEDTAFYRSARLLSRNDVGFEAERFSAPPMHFHNEAQRRLRDFPDNLLTTATHDHKRGE
DTRARLAVLSERGTWLASRVEHWRELAAPLRAQLDDGLAPSPGDELMLLQTLLGSWPLDL
DLNDDNALRQYAERVRQWQQKALREAKLRSSWSAPNEAYEGACAHYLDGLLLDSENQQLR
KSLADAAQLLACPGALNGLVQALLRMTTPGVPDLYQGNEYWDFSLVDPDNRRAVDYASRR
RTLDDATPAAELLAHWRDGRVKQALIARVLDCRQAHAELFRRGAYLPLTVQGRHADKVIA
FARLGEGEHAIVVAPRLASSLLGGAATPLIPAQNWDDTRLVLPFALSPANSTGLFACAAV
SSSKELPLSAVLAEFPVNVLIQQS