Protein Info for PP_4037 in Pseudomonas putida KT2440

Annotation: NADP-dependent dihydropyrimidine dehydrogenase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14691: Fer4_20
  • amino acids 20 to 127 (108 residues), 114.1 bits, E=1.5e-36
• PF03486: HI0933_like
  • amino acids 142 to 176 (35 residues), 23.6 bits, 1e-08
• PF01266: DAO
  • amino acids 142 to 176 (35 residues), 28.8 bits, 4.6e-10
• PF07992: Pyr_redox_2
  • amino acids 142 to 437 (296 residues), 126.8 bits, E=6e-40
• PF00890: FAD_binding_2
  • amino acids 143 to 177 (35 residues), 24.8 bits, 6.1e-09
• PF13450: NAD_binding_8
  • amino acids 145 to 177 (33 residues), 37.7 bits, see alignment (E = 1e-12)
• PF01593: Amino_oxidase
  • amino acids 151 to 177 (27 residues), 25.4 bits, see alignment (E = 4.5e-09)
• PF00070: Pyr_redox
  • amino acids 277 to 351 (75 residues), 22.5 bits, E=6.9e-08

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 100% identity to ppu:PP_4037)

Predicted SEED Role

"Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism" in subsystem Pyrimidine utilization

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Sequence Analysis Tools

See Q88FQ1 at UniProt or InterPro

Protein Sequence (455 amino acids)

>PP_4037 NADP-dependent dihydropyrimidine dehydrogenase subunit (Pseudomonas putida KT2440)
MTNALNHLPRPRAGADQLAERFSDLAPPLTARQAALESARCLYCYDAPCVNACPSEIDIP
SFIHRISDENLQGAAERILSANILGGSCARVCPTEILCQQACVRNNAQECAPVLIGQLQR
YALDNAQFSAHPFKRSAATGKRIAVVGAGPAGLSCAHRLAMHGHDVVVFEACDKAGGLNE
YGIARYKLVDDYAQREVDFLLGIGGIEVRHGQRLGGNLSLGELRDQYDAVFLGLGLNAVR
QLGLPDEEAPGVVAATEYIRELRQADDLSQLPLADRCLVIGAGNTAIDMAVQMSRLGARD
VNLVYRRGHADMGATGHEQDIAKANQVRLHTWARPDAVLLDDAGKVRGMRFARTELAEGR
LRDTGETFELAADAIFKAIGQRFDDASLGDPVAAQLAREGERIRVDENLQTSLPGVYAGG
DCTALGQDLTVQAVQHGKLAAEAIHAQLMLNVEAA