Protein Info for PP_4006 in Pseudomonas putida KT2440

Annotation: Putative arginyl-tRNA--protein transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 235 PF04376: ATE_N" amino acids 15 to 85 (71 residues), 88.7 bits, see alignment E=3.7e-29 PF13480: Acetyltransf_6" amino acids 81 to 206 (126 residues), 26.2 bits, see alignment E=1.2e-09 PF04377: ATE_C" amino acids 105 to 226 (122 residues), 147.9 bits, see alignment E=3.2e-47

Best Hits

Swiss-Prot: 100% identical to BPT_PSEPK: Aspartate/glutamate leucyltransferase (bpt) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00685, arginine-tRNA-protein transferase [EC: 2.3.2.8] (inferred from 100% identity to ppf:Pput_1827)

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.2.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FS6 at UniProt or InterPro

Protein Sequence (235 amino acids)

>PP_4006 Putative arginyl-tRNA--protein transferase (Pseudomonas putida KT2440)
MTELARLKFYATQPHSCSYLPEEQATTLFLDPSQPMDVHVYADLSEMGFRRSGDHLYRPH
CQNCNACVPARIPAARFIPNRQQRRILKRNADLTVTAARPAFKEEYFELYRRYIETRHAD
GDMYPPSRDQFSTFLVRDLPFCWFYEFRLEGRLMAVAVCDLLPNGLSAVYTFYEPDEERR
SLGRFAILWQITEALRQNLEAVYLGYWIKNCKKMNYKTQYRPIELLINQRWVTLN