Protein Info for PP_4005 in Pseudomonas putida KT2440

Annotation: Leucyl/phenylalanyl-tRNA--protein transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 TIGR00667: leucyl/phenylalanyl-tRNA--protein transferase" amino acids 32 to 217 (186 residues), 213.7 bits, see alignment E=8.2e-68 PF03588: Leu_Phe_trans" amino acids 34 to 203 (170 residues), 255 bits, see alignment E=2.7e-80 PF13480: Acetyltransf_6" amino acids 74 to 181 (108 residues), 23 bits, see alignment E=7.7e-09

Best Hits

Swiss-Prot: 100% identical to LFTR_PSEPK: Leucyl/phenylalanyl-tRNA--protein transferase (aat) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00684, leucyl/phenylalanyl-tRNA--protein transferase [EC: 2.3.2.6] (inferred from 100% identity to ppu:PP_4005)

MetaCyc: 51% identical to leucyl/phenylalanyl-tRNA--protein transferase (Escherichia coli K-12 substr. MG1655)
Leucyltransferase. [EC: 2.3.2.6]; 2.3.2.6 [EC: 2.3.2.6]; 2.3.2.6 [EC: 2.3.2.6]; 2.3.2.6 [EC: 2.3.2.6]

Predicted SEED Role

"Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6)" in subsystem Protein degradation (EC 2.3.2.6)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FS7 at UniProt or InterPro

Protein Sequence (226 amino acids)

>PP_4005 Leucyl/phenylalanyl-tRNA--protein transferase (Pseudomonas putida KT2440)
MLTWLTRDSLTFPPLEKALHDPNGLLAAGGDLSPERLVQAYRHGCFPWYQDGQPILWWSP
DPRTVLLPDQLHVSRSLAKLMRQGRYQVSFDTDFPAVIAACAAPRDYADGTWITDTMRNA
YCELHRRGIAHSVEVRQDGELVGGLYGLAMGQLFFGESMFSRADNASKVGFVTLVNHLRD
AGFVLIDCQMPTNHLHSLGAHAISRAEFASYLARHLDQPNSASWVA