Protein Info for PP_3999 in Pseudomonas putida KT2440

Annotation: Uroporphyrinogen-III C-methyltransferase/Precorrin-2 dehydrogenase/Sirohydrochlorin ferrochelatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 205 (202 residues), 252.3 bits, see alignment E=3.4e-79 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 91.7 bits, see alignment E=8.1e-30 PF14824: Sirohm_synth_M" amino acids 119 to 145 (27 residues), 31 bits, see alignment (E = 2.8e-11) PF10414: CysG_dimeriser" amino acids 150 to 206 (57 residues), 75.5 bits, see alignment 4.5e-25 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 216 to 451 (236 residues), 323 bits, see alignment E=1.4e-100 PF00590: TP_methylase" amino acids 218 to 427 (210 residues), 188.9 bits, see alignment E=2.2e-59

Best Hits

Swiss-Prot: 100% identical to CYSG_PSEPK: Siroheme synthase (cysG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to ppf:Pput_1834)

MetaCyc: 55% identical to uroporphyrinogen-III C-methyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Sirohydrochlorin ferrochelatase. [EC: 4.99.1.4]; Uroporphyrinogen-III C-methyltransferase. [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; Precorrin-2 dehydrogenase. [EC: 4.99.1.4, 2.1.1.107, 1.3.1.76]

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FT3 at UniProt or InterPro

Protein Sequence (463 amino acids)

>PP_3999 Uroporphyrinogen-III C-methyltransferase/Precorrin-2 dehydrogenase/Sirohydrochlorin ferrochelatase (Pseudomonas putida KT2440)
MDYLPLFHKLQGGRVLVVGGGEIALRKARLLADAGGVLRVVAPDVDGQLAALAREGGGEV
LVRGYQAADLVGCRLVIAATDDPGLNAQVSADAQALSLPVNVVDAPALCTVIFPAIVDRS
PLVIAVSSGGDAPVLARLIRAKLEAWIPSAYGELAGLAARFRHKVKSLYPDVNQRRGFWE
TVFQGPIAERQLAGQGAEAERLLQAMVDGAPVQQGGEVYLVGAGPGDPDLLTFRALRLMQ
QADVVLYDRLVAPAIIDMCRRDAERIYVGKRRADHSVPQDQINRLLVDLARQGKRVLRLK
GGDPFIFGRGGEEIEELAEHGIPFQVVPGITAASGCSAYGGIPLTHRDYAQSVRFVTGHL
KDGTSNLPWNDLVAPAQTLVFYMGLVGLPTICAELIRHGRAASTPAALVQQGTTRNQRVF
TGTLADLPDLVAQHEVHAPTLVIVGEVVQLRDKLAWFEGSQNS