Protein Info for PP_3970 in Pseudomonas putida KT2440

Annotation: putative oxidoreductase, Zn-dependent and NAD(P)-binding

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 PF08240: ADH_N" amino acids 25 to 153 (129 residues), 86.8 bits, see alignment E=1.1e-28 PF00107: ADH_zinc_N" amino acids 197 to 285 (89 residues), 38.1 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 73% identical to YBDR_ECOLI: Uncharacterized zinc-type alcohol dehydrogenase-like protein YbdR (ybdR) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3970)

Predicted SEED Role

"Uncharacterized zinc-type alcohol dehydrogenase-like protein ybdR"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FV8 at UniProt or InterPro

Protein Sequence (400 amino acids)

>PP_3970 putative oxidoreductase, Zn-dependent and NAD(P)-binding (Pseudomonas putida KT2440)
MRAVSYHGANDVRVDSVPDPILQDADDIILRVTATAICGSDLHLYRGKIPETEQGDIFGH
EFMGIVEEVGRDVTTLQVGDRVVIPFVIACGSCFFCQQDLFAACETTNTGRGSIINKKGI
PPGAALFGYSHLYGGIPGGQADYVRVPKGNVGPFKVPTTLADDKVLFLSDILPTAWQAVI
NAEIGEGSSVAIYGAGPVGLLSAACARMLGAHTVFMVDDNDYRLAYAQEAYGVIPINFEK
DDDPADSIIRQTPGMRGVDAVIDAVGFEAKGSTTETVMTALKLEGSSGKALRQSIAAVRR
GGVVSVPGVYAGFIHGFMFGDAFDKGLTFKMGQTHVQKYLPELLEHIEAGRLQPELIVTH
RLALEEAAMGYKMFDQKQDNCRKVILVPGAAPGTLGPDHL