Protein Info for PP_3957 in Pseudomonas putida KT2440

Annotation: choline/carnitine/betaine transporter BetTC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 transmembrane" amino acids 15 to 33 (19 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 144 to 164 (21 residues), see Phobius details amino acids 185 to 211 (27 residues), see Phobius details amino acids 231 to 251 (21 residues), see Phobius details amino acids 263 to 284 (22 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 347 to 366 (20 residues), see Phobius details amino acids 403 to 426 (24 residues), see Phobius details amino acids 446 to 465 (20 residues), see Phobius details amino acids 472 to 492 (21 residues), see Phobius details PF02028: BCCT" amino acids 15 to 496 (482 residues), 661.2 bits, see alignment E=4.2e-203 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 53 to 497 (445 residues), 542.9 bits, see alignment E=3e-167

Best Hits

Swiss-Prot: 53% identical to GBTR_ACIAD: Glycine betaine transporter (ACIAD3460) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 100% identity to ppu:PP_3957)

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FX0 at UniProt or InterPro

Protein Sequence (657 amino acids)

>PP_3957 choline/carnitine/betaine transporter BetTC (Pseudomonas putida KT2440)
MTAAPKPRSTLNPPVFYTSAILVFLLVLYATAFQEHAQTLFEQVQRWIITNASWFYILVV
ALVLISVVFLAVSRYGDIKLGPDHSEPDYRKSSWFAMLFSAGMGIGLMFFGVAEPVMHFS
TPPVGDPGTVAAAREAMKITFFHWGLHAWAIYAIVALILAYFSFRNGLPLTLRSALYPLI
GERIYGPIGHAVDVFAILGTVFGVATSLGYGVLQINSGFHHVFGLPVNTTVQVILITATC
ALATLSVASGLDKGIRILSELNLSLAVILMLFVLLLGPTVFLLQTYVQNTGAYLSDIVNK
TFNLYAYEPTDWIGGWTLLYWGWWLSWSPFVGLFIARISRGRTIREFVCGVLFVPAGFTL
LWMTVFGDSAIHMILNDGVKDLAAVVSEDSSLALFAFLEHFPFSSIISLVAVLMVVVFFV
TSADSGALVVDMLASSGQGHSPLWQRIFWSVCIGAVAIALLLANGLKALQTATIASALPF
AVILLVAIWGLFKALSLDATRRGLRNQALPGPRHTRHPHGGWQRRLRNIALMPRRVHVTR
FIAEVVRPACEEVAQELRKQGYEVSVNERDDGRVSLELSHAGEGRFLYEVRPRAFNTPSF
VMRDTEDGADSRKYFRAEVHLREGGQDYDIMGWSRDDVIGDILDQYERHLHYLHVVG