Protein Info for PP_3747 in Pseudomonas putida KT2440

Annotation: glycolate oxidase, iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 PF13237: Fer4_10" amino acids 22 to 86 (65 residues), 29.6 bits, see alignment E=2.1e-10 PF13183: Fer4_8" amino acids 22 to 89 (68 residues), 46 bits, see alignment E=2.6e-15 PF12838: Fer4_7" amino acids 24 to 89 (66 residues), 41.6 bits, see alignment E=5.5e-14 PF13534: Fer4_17" amino acids 25 to 89 (65 residues), 41.8 bits, see alignment E=5.3e-14 PF02754: CCG" amino acids 172 to 255 (84 residues), 48.4 bits, see alignment E=3.4e-16 amino acids 303 to 386 (84 residues), 47.6 bits, see alignment E=6e-16

Best Hits

Swiss-Prot: 73% identical to GLCF_ECOLI: Glycolate oxidase iron-sulfur subunit (glcF) from Escherichia coli (strain K12)

KEGG orthology group: K11473, glycolate oxidase iron-sulfur subunit (inferred from 100% identity to ppu:PP_3747)

MetaCyc: 73% identical to glycolate dehydrogenase, putative iron-sulfur subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.14

Use Curated BLAST to search for 1.1.99.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GH6 at UniProt or InterPro

Protein Sequence (411 amino acids)

>PP_3747 glycolate oxidase, iron-sulfur subunit (Pseudomonas putida KT2440)
MQTNLSETSRRLARADEAERILRTCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLE
GEPVTASTQLHLDRCLTCRNCETTCPSGVKYHDLLDIGRAVVEQQVPRPLGQRLLRLGLR
AVVPRPALFKALTRSGQVLRPLLPASVKRKLPAQVTTPGQRPAVRHARRILMLEGCVQPA
LSPNTNAAAARLLDRLGISVQPVRQAGCCGAVDYHLNAQEQGLQRARRNIDAWWPAIEAG
VEAIVQTASGCGAFVRDYGHLLEKDPHYAAKAARVSALSRDLVEILRDEPIEQLALCAEQ
RLAFHCPCTLQHALKLGGAVEALLTRLGFTLTSVPDGHLCCGSAGTYSLTQPELSLQLRD
NRLNALESGKPHLIATANIGCQAHLDGAGRTPVRHWVEIVEAALNPEPAHA