Protein Info for PP_3725 in Pseudomonas putida KT2440

Annotation: putative Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 PF02771: Acyl-CoA_dh_N" amino acids 6 to 117 (112 residues), 69 bits, see alignment E=9.3e-23 PF02770: Acyl-CoA_dh_M" amino acids 121 to 220 (100 residues), 89.8 bits, see alignment E=2.1e-29 PF00441: Acyl-CoA_dh_1" amino acids 232 to 381 (150 residues), 177.5 bits, see alignment E=3.9e-56 PF08028: Acyl-CoA_dh_2" amino acids 248 to 369 (122 residues), 85.9 bits, see alignment E=6.1e-28

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppg:PputGB1_2181)

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GJ7 at UniProt or InterPro

Protein Sequence (386 amino acids)

>PP_3725 putative Acyl-CoA dehydrogenase (Pseudomonas putida KT2440)
MNFQLTQEQEMLVEAVRSFVAKELLPHEEAVDRADAVSPELAAQIRGKAIAAGFYAFNMP
EEVGGGGLDYLSQALIERELSKVSWALHVFVARPSKILMACKDEQINDYLLPCVQGEKVD
CFALTEPGAGSDANAIKTRAVRQGDDFVINGSKHFISHAGHADFAIVFAVTDTYEHNGRK
RNAVTALLVDRGTPGMTIRRGPKCVSNRGYHTYELFFDDCRVPASKVLGEVGKGWEVANA
WLTAGRVMVAANCVGQAQRALDLSLQWAADRKQFGQAIGSYQGVSFKLADMATQIRAAEM
LTLHTAWKMDQGNMTDGEAGMAKLFASEVLGKVADEAVQIFGGMGLMDEGPVERIWRNAR
IERIWEGTSEIQRHIIARELLRPLLR