Protein Info for PP_3723 in Pseudomonas putida KT2440
Annotation: putative 2-ketoarginine decarboxylase AruI
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to ARUI_PSEAE: Probable 2-ketoarginine decarboxylase AruI (aruI) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K12253, 5-guanidino-2-oxopentanoate decarboxylase [EC: 4.1.1.75] (inferred from 95% identity to ppf:Pput_2040)MetaCyc: 82% identical to 2-ketoarginine decarboxylase (Pseudomonas aeruginosa)
5-guanidino-2-oxopentanoate decarboxylase. [EC: 4.1.1.75]
Predicted SEED Role
"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-arginine degradation IX (arginine:pyruvate transaminase pathway) (4/4 steps found)
- L-valine biosynthesis (4/4 steps found)
- pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
- L-arginine degradation VIII (arginine oxidase pathway) (3/4 steps found)
- pyruvate fermentation to (R)-acetoin I (2/3 steps found)
- pyruvate fermentation to (S)-acetoin (2/3 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- pyruvate fermentation to (R)-acetoin II (1/2 steps found)
- superpathway of (R,R)-butanediol biosynthesis (3/5 steps found)
- L-isoleucine biosynthesis II (5/8 steps found)
- L-arginine degradation XII (2/4 steps found)
- superpathway of L-threonine metabolism (12/18 steps found)
- L-isoleucine biosynthesis III (4/7 steps found)
- superpathway of 2,3-butanediol biosynthesis (2/6 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Butanoate metabolism
- C5-Branched dibasic acid metabolism
- Pantothenate and CoA biosynthesis
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.2.1.6
Use Curated BLAST to search for 2.2.1.6 or 4.1.1.75
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A140FWH9 at UniProt or InterPro
Protein Sequence (535 amino acids)
>PP_3723 putative 2-ketoarginine decarboxylase AruI (Pseudomonas putida KT2440) MNDLTLTAGQALVRLLANYGVETVFGIPGVHTLELYRGLPGSGIRHVLTRHEQGAGFMAD GYARVSGKPGVCFVITGPGVTNVATPIGQAYADSVPMLVISSVNHTASLGKGWGCLHETQ DQRAMTAPITAFSAVALQGDDLPELIARAWAVFDSERPRPVHISVPLDVLAASVSRDWSG EVVRRPGRGQPCRETLEQAALKLAAAQRPMIIAGGGALHAAEQLQQLSTRLAAPLFTSVA GKGLLPPDAPLNAGASLCVEPGWQLISQADVVLAVGTEMADTDFWRERLPIRGELLRVDI DPRKFNDFYPCAIALQGDARQTLDGLLEHLPALQRDPAQASAAVATLRQAIRNGHAPLQA THQAILDRIAAVLPDDAFISSDMTQLAYTGNYAFASRAPRSWLHPTGYGTLGYGLPAGIG GMFASDHRPGLVLVGDGGFLYTAQELATAVEELHRPLVVLLWNNDALGQIRDDMLGLDIE PVGVLPRNPDFIGLARAFGCTVHQPRDLDALQADLASGFATPGVTFIELKHTCVC