Protein Info for PP_3721 in Pseudomonas putida KT2440
Updated annotation (from data): arginine:pyruvate transaminase AruH (EC 2.6.1.84)
Rationale: Specific phenotype: utilization of L-Arginine. Similar to P.aeruginosa aruH (Q9HUI9)
Original annotation: Arginine--pyruvate transaminase AruH
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 81% identical to ARUH_PSEAE: Arginine--pyruvate transaminase AruH (aruH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K12252, arginine:pyruvate transaminase [EC: 2.6.1.84] (inferred from 100% identity to ppu:PP_3721)MetaCyc: 81% identical to L-arginine:pyruvate transaminase subunit (Pseudomonas aeruginosa)
Arginine--pyruvate transaminase. [EC: 2.6.1.84]
Predicted SEED Role
"Valine--pyruvate aminotransferase (EC 2.6.1.66)" in subsystem Alanine biosynthesis or Branched-Chain Amino Acid Biosynthesis or Pyruvate Alanine Serine Interconversions (EC 2.6.1.66)
MetaCyc Pathways
- L-arginine degradation IX (arginine:pyruvate transaminase pathway) (4/4 steps found)
- superpathway of L-alanine biosynthesis (4/4 steps found)
- L-alanine biosynthesis I (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.66
Use Curated BLAST to search for 2.6.1.66 or 2.6.1.84
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88GK0 at UniProt or InterPro
Protein Sequence (396 amino acids)
>PP_3721 arginine:pyruvate transaminase AruH (EC 2.6.1.84) (Pseudomonas putida KT2440) MRYAKLTQRIAGDGAAAWDIHYRALALQAEDKDILLLSVGDPDFDTPAPIVEAAIDSLRA GHTHYADVRGKLALRQAIANRHRQRSGQAVSADQVTVLAGAQCALYCVAQCILDPGDEVI VAEPMYVTYEAVFGACGAKVVPVPVKPENGFRVCPRDVAERITPRTRALALNSPHNPSGA SLPRATWEALAELCVAHDLWLISDEVYSELLYEGEHVSPGSLPGMAERTATLNSLSKSHA MTGWRMGWVVGSTALATHLENLALCMLYGLPDFVQDAAVVALEHPLPELDAMREAYRQRR DLVCEQLAGCPGLKALKPDGGMFVMLDIRETGVSAQAFADYLLDSQGVSVLAGEAFGPSA AGHIRLGLVLGNEALVDACQRIARCAGELMRGQTDA